Restriction map for
pFN21AE3250
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BseRI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 839 CGCG CG^CG
AcyI 649 GACGCC G[GA]^CG[CT]C
AflII 1441 CTTAAG C^TTAAG
AflIII 454 ACATGT A^C[GA][CT]GT
AlwI 180 GGATC GGATC(4/5)
Asp700I 612 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 623 TGGCCA TGG^CCA
BanI 519 GGCACC G^G[CT][GA]CC
BfrI 1441 CTTAAG C^TTAAG
BmyI 981 GTGCCC G[AGT]GC[ACT]^C
BsaHI 649 GACGCC G[GA]^CG[CT]C
BsaWI 1283 ACCGGA [AT]^CCGG[AT]
BsiEI 65 CGACCG CG[GA][CT]^CG
BsmAI 1287 GAGAC GTCTC(1/5)
BsmFI 77 GGGAC GGGAC(10/14)
Bsp1286I 981 GTGCCC G[AGT]GC[ACT]^C
BspHI 1227 TCATGA T^CATGA
BspLU11I 454 ACATGT A^CATGT
BspMI 595 ACCTGC ACCTGC(4/8)
BstF5I 59 GGATG GGATG(2/0)
BstUI 839 CGCG CG^CG
BstYI 180 GGATCT [GA]^GATC[CT]
DraII 77 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 186 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 77 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 423 CCAAGG C^C[AT][AT]GG
FokI 59 GGATG GGATG(9/13)
HgaI 649 GACGC GACGC(5/10)
Hin1I 649 GACGCC G[GA]^CG[CT]C
HphI 1278 TCACC GGTGA(8/7)
MaeII 244 ACGT A^CGT
MflI 180 GGATCT [GA]^GATC[CT]
MluNI 623 TGGCCA TGG^CCA
MscI 623 TGGCCA TGG^CCA
MslI 451 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 839 CGCG CG^CG
PpuMI 77 GGGACCC [GA]G^G[AT]CC[CT]
PstI 597 CTGCAG CTGCA^G
RcaI 1227 TCATGA T^CATGA
SbfI 596 CCTGCAGG CCTGCA^GG
SmaI 15 CCCGGG CCC^GGG
Sse8387I 596 CCTGCAGG CCTGCA^GG
StyI 423 CCAAGG C^C[AT][AT]GG
TaqI 215 TCGA T^CGA
TfiI 785 GAATC G^A[AT]TC
Tsp45I 136 GTGAC ^GT[GC]AC
TthHB8I 215 TCGA T^CGA
XhoII 180 GGATCT [GA]^GATC[CT]
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
XmnI 612 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcsI 302 GAATTT [GA]^AATT[CT]
913 AAATTC
ApoI 302 GAATTT [GA]^AATT[CT]
913 AAATTC
AvaI 4 CTCGGG C^[CT]CG[GA]G
15 CCCGGG
BfaI 794 CTAG C^TAG
1604 CTAG
BsoBI 4 CTCGGG C^[CT]CG[GA]G
15 CCCGGG
BsrDI 899 GCAATG GCAATG(2/0)
1235 GCAATG
EaeI 152 TGGCCG [CT]^GGCC[GA]
623 TGGCCA
FauI 24 CCCGC CCCGC(4/6)
437 GCGGG
MaeI 794 CTAG C^TAG
1604 CTAG
MwoI 495 GCACCAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1019 GCCTCAATAGC
NspI 454 ACATGT [GA]CATG^[CT]
832 GCATGT
PleI 54 GACTC GAGTC(4/5)
367 GACTC
SfaNI 222 GATGC GCATC(5/9)
1113 GCATC
SfcI 101 CTACAG C^T[GA][CT]AG
597 CTGCAG
SmlI 393 CTTGAG C^T[CT][GA]AG
1441 CTTAAG
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BpmI BsaJI BsgI BsiYI
BslI BsmI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MseI MspI MvaI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI StuI Tru9I TseI Tsp509I
TspRI