Restriction map for
pFN21AE2511
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HgaI
HindIII HpaI HphI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MluNI MscI MseI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 791 TCCGGA T^CCGGA
AcyI 314 GGCGCC G[GA]^CG[CT]C
AlwNI 670 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 341 AGCGCT AGC^GCT
AviII 548 TGCGCA TGC^GCA
BamHI 686 GGATCC G^GATCC
BbeI 314 GGCGCC GGCGC^C
BlpI 376 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 376 GCTAAGC GC^T[ACGT]AGC
BsaHI 314 GGCGCC G[GA]^CG[CT]C
BsaWI 791 TCCGGA [AT]^CCGG[AT]
BseAI 791 TCCGGA T^CCGGA
BspDI 244 ATCGAT AT^CGAT
BspEI 791 TCCGGA T^CCGGA
BspMI 221 ACCTGC ACCTGC(4/8)
BsrBI 310 GAGCGG CCGCTC(-3/-3)
BsrI 683 ACTGG ACTGG(1/-1)
CelII 376 GCTAAGC GC^T[ACGT]AGC
ClaI 244 ATCGAT AT^CGAT
DraII 472 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 134 CCGTGG C^C[GA][CT]GG
Eco47III 341 AGCGCT AGC^GCT
EcoNI 492 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 472 GGGGCCC [GA]G^G[ACGT]CC[CT]
FspI 548 TGCGCA TGC^GCA
Hin1I 314 GGCGCC G[GA]^CG[CT]C
HincII 181 GTTGAC GT[CT]^[GA]AC
HindII 181 GTTGAC GT[CT]^[GA]AC
HinfI 700 GAGTC G^A[ACGT]TC
KasI 314 GGCGCC G^GCGCC
MroI 791 TCCGGA T^CCGGA
NarI 314 GGCGCC GG^CGCC
PflMI 690 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 700 GAGTC GAGTC(4/5)
PstI 232 CTGCAG CTGCA^G
PvuII 694 CAGCTG CAG^CTG
SapI 79 GAAGAGC GCTCTTC(1/4)
SexAI 491 ACCTGGT A^CC[AT]GGT
SfcI 232 CTGCAG C^T[GA][CT]AG
SfoI 314 GGCGCC GGC^GCC
Van91I 690 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 250 GTAC GT^AC
762 GTAC
ApaI 473 GGGCCC GGGCC^C
611 GGGCCC
BanI 138 GGTGCC G^G[CT][GA]CC
314 GGCGCC
BanII 473 GGGCCC G[GA]GC[CT]^C
611 GGGCCC
BcnI 609 CCGGG CC^[GC]GG
614 CCCGG
BmyI 473 GGGCCC G[AGT]GC[ACT]^C
611 GGGCCC
BpmI 226 CTGGAG CTGGAG(16/14)
697 CTGGAG
Bsp1286I 473 GGGCCC G[AGT]GC[ACT]^C
611 GGGCCC
Bsp1407I 249 TGTACA T^GTACA
761 TGTACA
BsrFI 90 GCCGGT [GA]^CCGG[CT]
317 GCCGGT
BsrGI 249 TGTACA T^GTACA
761 TGTACA
BstYI 161 AGATCC [GA]^GATC[CT]
686 GGATCC
Cfr10I 90 GCCGGT [GA]^CCGG[CT]
317 GCCGGT
HaeII 314 GGCGCC [GA]GCGC^[CT]
341 AGCGCT
MaeII 369 ACGT A^CGT
755 ACGT
MflI 161 AGATCC [GA]^GATC[CT]
686 GGATCC
NciI 609 CCGGG CC^[GC]GG
614 CCCGG
RsaI 250 GTAC GT^AC
762 GTAC
SspBI 249 TGTACA T^GTACA
761 TGTACA
XhoII 161 AGATCC [GA]^GATC[CT]
686 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BsaJI BseRI BsiYI BslI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TthHB8I