Restriction map for
pFN21AE4027
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeIII HphI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 672 CGCG CG^CG
AcsI 404 GAATTC [GA]^AATT[CT]
AcyI 542 GGCGTC G[GA]^CG[CT]C
AhdI 614 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 160 GATCC GGATC(4/5)
Aor51HI 463 AGCGCT AGC^GCT
ApoI 404 GAATTC [GA]^AATT[CT]
Asp700I 400 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 614 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 266 GAGCTC G[AT]GC[AT]^C
BanII 266 GAGCTC G[GA]GC[CT]^C
BciVI 186 GTATCC GTATCC(6/5)
BcnI 17 CCCGG CC^[GC]GG
BglI 534 GCCGCTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 453 AGATCT A^GATCT
BmyI 266 GAGCTC G[AGT]GC[ACT]^C
BsaHI 542 GGCGTC G[GA]^CG[CT]C
BsgI 666 GTGCAG GTGCAG(16/14)
BsiHKAI 266 GAGCTC G[AT]GC[AT]^C
Bsp1286I 266 GAGCTC G[AGT]GC[ACT]^C
BsrDI 506 CATTGC GCAATG(2/0)
BsrI 143 ACTGG ACTGG(1/-1)
BstF5I 41 GGATG GGATG(2/0)
BstNI 190 CCAGG CC^[AT]GG
BstUI 672 CGCG CG^CG
Eam1105I 614 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 463 AGCGCT AGC^GCT
EcoRI 404 GAATTC G^AATTC
EcoRII 190 CCAGG ^CC[AT]GG
FokI 41 GGATG GGATG(9/13)
HaeII 463 AGCGCT [GA]GCGC^[CT]
HapII 18 CCGG C^CGG
HgaI 543 GCGTC GACGC(5/10)
Hin1I 542 GGCGTC G[GA]^CG[CT]C
HindIII 559 AAGCTT A^AGCTT
HpaI 52 GTTAAC GTT^AAC
HpaII 18 CCGG C^CGG
MaeII 380 ACGT A^CGT
MaeIII 622 GTCAC ^GT[ACGT]AC
MspI 18 CCGG C^CGG
MvaI 190 CCAGG CC^[AT]GG
MvnI 672 CGCG CG^CG
MwoI 534 GCCGCTAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 17 CCCGG CC^[GC]GG
NheI 135 GCTAGC G^CTAGC
NspI 174 GCATGC [GA]CATG^[CT]
PshAI 652 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 266 GAGCTC GAGCT^C
SapI 529 GAAGAGC GCTCTTC(1/4)
SphI 174 GCATGC GCATG^C
Tsp45I 622 GTCAC ^GT[GC]AC
XmnI 400 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 535 CCGC CCGC(-3/-1)
673 GCGG
BbvI 63 GCAGC GCAGC(8/12)
668 GCAGC
BsaJI 152 CCAAGG C^C[ACGT][ACGT]GG
163 CCTTGG
BseRI 128 GAGGAG GAGGAG(10/8)
553 GAGGAG
BssKI 17 CCCGG ^CC[ACGT]GG
190 CCAGG
BstYI 159 AGATCC [GA]^GATC[CT]
453 AGATCT
CfoI 464 GCGC GCG^C
671 GCGC
DdeI 467 CTCAG C^T[ACGT]AG
538 CTAAG
DpnII 160 GATC ^GATC
454 GATC
EarI 211 GAAGAG CTCTTC(1/4)
529 GAAGAG
EcoT14I 152 CCAAGG C^C[AT][AT]GG
163 CCTTGG
HhaI 464 GCGC GCG^C
671 GCGC
HinP1I 464 GCGC G^CGC
671 GCGC
HincII 52 GTTAAC GT[CT]^[GA]AC
649 GTTGAC
HindII 52 GTTAAC GT[CT]^[GA]AC
649 GTTGAC
HinfI 311 GAATC G^A[ACGT]TC
495 GAATC
Ksp632I 211 GAAGAG CTCTTC(1/4)
529 GAAGAG
MboI 160 GATC ^GATC
454 GATC
MflI 159 AGATCC [GA]^GATC[CT]
453 AGATCT
NdeII 160 GATC ^GATC
454 GATC
Sau3AI 160 GATC ^GATC
454 GATC
ScrFI 17 CCCGG CC^[ACGT]GG
190 CCAGG
SfcI 89 CTATAG C^T[GA][CT]AG
479 CTACAG
SmlI 259 CTTGAG C^T[CT][GA]AG
436 CTTGAG
StyI 152 CCAAGG C^C[AT][AT]GG
163 CCTTGG
TfiI 311 GAATC G^A[AT]TC
495 GAATC
TseI 63 GCAGC G^C[AT]GC
668 GCAGC
Tsp509I 405 AATT ^AATT
491 AATT
XhoII 159 AGATCC [GA]^GATC[CT]
453 AGATCT
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AfaI AluI BfaI BsmFI Cac8I
Fnu4HI ItaI MaeI MboII MnlI
MseI NlaIII RsaI Tru9I TspRI