Restriction map for
pFN21AE3096
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI ApaI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI Ksp632I
KspI MamI MboII MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 518 GGTACC G^GTACC
AccI 745 GTCTAC GT^[AC][GT]AC
AccII 325 CGCG CG^CG
Alw44I 138 GTGCAC G^TGCAC
Aor51HI 716 AGCGCT AGC^GCT
ApaLI 138 GTGCAC G^TGCAC
Asp718I 518 GGTACC G^GTACC
AvaI 463 CCCGGG C^[CT]CG[GA]G
BbvCI 35 CCTCAGC CCTCAGC(-5/-2)
BfaI 587 CTAG C^TAG
BglI 169 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 688 CTCCAG CTGGAG(16/14)
BsaAI 135 TACGTG [CT]AC^GT[GA]
BseRI 294 GAGGAG GAGGAG(10/8)
BsiEI 252 CGGCCG CG[GA][CT]^CG
BsoBI 463 CCCGGG C^[CT]CG[GA]G
BspMI 315 GCAGGT ACCTGC(4/8)
BsrDI 534 GCAATG GCAATG(2/0)
BsrI 225 CCAGT ACTGG(1/-1)
BssSI 697 CTCGTG CACGAG(-5/-1)
BstAPI 315 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 441 GGTCACC G^GT[ACGT]ACC
BstPI 441 GGTCACC G^GT[ACGT]ACC
BstUI 325 CGCG CG^CG
DsaI 157 CCGTGG C^C[GA][CT]GG
EaeI 252 CGGCCG [CT]^GGCC[GA]
EagI 252 CGGCCG C^GGCCG
EclXI 252 CGGCCG C^GGCCG
Eco47III 716 AGCGCT AGC^GCT
Eco52I 252 CGGCCG C^GGCCG
EcoO65I 441 GGTCACC G^GT[ACGT]ACC
HgaI 342 GACGC GACGC(5/10)
KpnI 518 GGTACC GGTAC^C
MaeI 587 CTAG C^TAG
MaeII 136 ACGT A^CGT
MvnI 325 CGCG CG^CG
NotI 251 GCGGCCGC GC^GGCCGC
PshAI 640 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 391 CTGCAG CTGCA^G
SacI 147 GAGCTC GAGCT^C
SfaNI 789 GATGC GCATC(5/9)
SmaI 463 CCCGGG CCC^GGG
Tsp509I 123 AATT ^AATT
XmaCI 463 CCCGGG C^CCGGG
XmaI 463 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 188 GGCGCC G[GA]^CG[CT]C
208 GGCGCC
AfaI 519 GTAC GT^AC
765 GTAC
AlwI 261 GATCC GGATC(4/5)
413 GGATC
AlwNI 316 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
768 CAGAAGCTG
AocI 6 CCTGAGG CC^T[ACGT]AGG
291 CCTGAGG
AspHI 138 GTGCAC G[AT]GC[AT]^C
147 GAGCTC
BanII 18 GGGCTC G[GA]GC[CT]^C
147 GAGCTC
BbeI 188 GGCGCC GGCGC^C
208 GGCGCC
BsaHI 188 GGCGCC G[GA]^CG[CT]C
208 GGCGCC
BsgI 614 GTGCAG GTGCAG(16/14)
675 GTGCAG
BsiHKAI 138 GTGCAC G[AT]GC[AT]^C
147 GAGCTC
BstF5I 108 GGATG GGATG(2/0)
561 GGATG
Bsu36I 6 CCTGAGG CC^T[ACGT]AGG
291 CCTGAGG
DpnII 261 GATC ^GATC
414 GATC
Eco81I 6 CCTGAGG CC^T[ACGT]AGG
291 CCTGAGG
FokI 108 GGATG GGATG(9/13)
561 GGATG
Hin1I 188 GGCGCC G[GA]^CG[CT]C
208 GGCGCC
HinfI 640 GACTC G^A[ACGT]TC
655 GACTC
KasI 188 GGCGCC G^GCGCC
208 GGCGCC
MboI 261 GATC ^GATC
414 GATC
NarI 188 GGCGCC GG^CGCC
208 GGCGCC
NdeII 261 GATC ^GATC
414 GATC
PleI 640 GACTC GAGTC(4/5)
655 GACTC
RsaI 519 GTAC GT^AC
765 GTAC
Sau3AI 261 GATC ^GATC
414 GATC
SfoI 188 GGCGCC GGC^GCC
208 GGCGCC
StuI 449 AGGCCT AGG^CCT
578 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PpuMI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI