##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       BalI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MunI        
MvnI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AflIII        599  ACATGT               A^C[GA][CT]GT
BamHI         770  GGATCC               G^GATCC
BbsI           66  GTCTTC               GAAGAC(2/6)
BcnI          396  CCCGG                CC^[GC]GG
BpmI          382  CTGGAG               CTGGAG(16/14)
BpuAI          66  GTCTTC               GAAGAC(2/6)
BsaWI         335  ACCGGA               [AT]^CCGG[AT]
BseRI         559  GAGGAG               GAGGAG(10/8)
BsmBI         693  CGTCTC               CGTCTC(1/5)
BspLU11I      599  ACATGT               A^CATGT
BsrI           95  CCAGT                ACTGG(1/-1)
BstYI         770  GGATCC               [GA]^GATC[CT]
MaeII         761  ACGT                 A^CGT
MboII          67  TCTTC                GAAGA(8/7)
MflI          770  GGATCC               [GA]^GATC[CT]
MslI          361  CACGACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          610  GCCGGC               GCC^GGC
NciI          396  CCCGG                CC^[GC]GG
NgoMIV        610  GCCGGC               G^CCGGC
NspI          599  ACATGT               [GA]CATG^[CT]
PpuMI         209  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          388  CTGCAG               CTGCA^G
SfaNI         689  GCATC                GCATC(5/9)
SfcI          388  CTGCAG               C^T[GA][CT]AG
SmlI          753  CTCAAG               C^T[CT][GA]AG
SpeI          159  ACTAGT               A^CTAGT
Tsp45I        647  GTGAC                ^GT[GC]AC
Tsp509I       511  AATT                 ^AATT
XhoII         770  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccI          574  GTCTAC               GT^[AC][GT]AC
              739  GTCTAC              
AlwI          771  GATCC                GGATC(4/5)
              770  GGATC               
ApaI          657  GGGCCC               GGGCC^C
              822  GGGCCC              
AvrII         255  CCTAGG               C^CTAGG
              270  CCTAGG              
BanI          460  GGCACC               G^G[CT][GA]CC
              487  GGCACC              
BlnI          255  CCTAGG               C^CTAGG
              270  CCTAGG              
BsmFI          91  GGGAC                GGGAC(10/14)
              393  GTCCC               
BspMI         121  ACCTGC               ACCTGC(4/8)
              294  GCAGGT              
BsrFI         610  GCCGGC               [GA]^CCGG[CT]
              728  ACCGGC              
BstF5I        417  CATCC                GGATG(2/0)
              540  CATCC               
Cfr10I        610  GCCGGC               [GA]^CCGG[CT]
              728  ACCGGC              
DdeI          137  CTCAG                C^T[ACGT]AG
              552  CTCAG               
FokI          417  CATCC                GGATG(9/13)
              540  CATCC               
MaeIII        647  GTGAC                ^GT[ACGT]AC
              671  GTAAC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstNI       Cac8I       
Cfr13I      DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeI        MboI        
MnlI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       StyI        TseI        TspRI