Restriction map for
pFN21AE6258
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 694 GTCTAC GT^[AC][GT]AC
AccII 128 CGCG CG^CG
AluI 421 AGCT AG^CT
AlwNI 263 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 612 GGGCCC GGGCC^C
AspHI 508 GAGCAC G[AT]GC[AT]^C
BanI 415 GGCACC G^G[CT][GA]CC
BanII 612 GGGCCC G[GA]GC[CT]^C
BbsI 123 GAAGAC GAAGAC(2/6)
BpmI 229 CTCCAG CTGGAG(16/14)
BpuAI 123 GAAGAC GAAGAC(2/6)
BsaI 55 GAGACC GGTCTC(1/5)
BsgI 571 GTGCAG GTGCAG(16/14)
BsiHKAI 508 GAGCAC G[AT]GC[AT]^C
BsmBI 733 GAGACG CGTCTC(1/5)
BsrDI 323 GCAATG GCAATG(2/0)
BstAPI 416 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 128 CGCG CG^CG
CfoI 488 GCGC GCG^C
DdeI 269 CTGAG C^T[ACGT]AG
DraIII 398 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 554 CCATGG C^C[GA][CT]GG
EarI 635 CTCTTC CTCTTC(1/4)
EcoNI 228 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 554 CCATGG C^C[AT][AT]GG
FauI 197 CCCGC CCCGC(4/6)
HhaI 488 GCGC GCG^C
HinP1I 488 GCGC G^CGC
Ksp632I 635 CTCTTC CTCTTC(1/4)
MaeII 448 ACGT A^CGT
MseI 16 TTAA T^TAA
MspA1I 420 CAGCTG C[AC]G^C[GT]G
MvnI 128 CGCG CG^CG
NcoI 554 CCATGG C^CATGG
PflMI 362 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 10 CTGCAG CTGCA^G
PvuII 420 CAGCTG CAG^CTG
SfcI 10 CTGCAG C^T[GA][CT]AG
SmlI 190 CTCAAG C^T[CT][GA]AG
StyI 554 CCATGG C^C[AT][AT]GG
TfiI 6 GATTC G^A[AT]TC
Tru9I 16 TTAA T^TAA
Van91I 362 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BamHI 663 GGATCC G^GATCC
725 GGATCC
BmyI 508 GAGCAC G[AGT]GC[ACT]^C
612 GGGCCC
BseRI 393 CTCCTC GAGGAG(10/8)
238 GAGGAG
Bsp1286I 508 GAGCAC G[AGT]GC[ACT]^C
612 GGGCCC
BstYI 663 GGATCC [GA]^GATC[CT]
725 GGATCC
DraII 611 GGGGCCC [GA]G^G[ACGT]CC[CT]
612 GGGCCCT
EcoO109I 611 GGGGCCC [GA]G^G[ACGT]CC[CT]
612 GGGCCCT
HaeIII 613 GGCC GG^CC
658 GGCC
HgaI 126 GACGC GACGC(5/10)
147 GACGC
MflI 663 GGATCC [GA]^GATC[CT]
725 GGATCC
NlaIII 333 CATG CATG^
555 CATG
SfaNI 485 GATGC GCATC(5/9)
349 GCATC
TaqI 77 TCGA T^CGA
119 TCGA
Tsp45I 406 GTCAC ^GT[GC]AC
602 GTGAC
TthHB8I 77 TCGA T^CGA
119 TCGA
XhoII 663 GGATCC [GA]^GATC[CT]
725 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AlwI BbvI BcnI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstNI Cac8I Cfr13I DpnII EcoRII
Fnu4HI HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIV PleI Sau3AI Sau96I
ScrFI TseI TspRI