Restriction map for
pFN21AE6266
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaII AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsgI BsiEI BsiHKAI BsmFI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
Ksp632I MaeI MamI MboII MfeI
MluI MseI MslI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 38 GGTACC G^GTACC
AccI 706 GTCTAC GT^[AC][GT]AC
AccII 429 CGCG CG^CG
AccIII 395 TCCGGA T^CCGGA
AcsI 724 AAATTC [GA]^AATT[CT]
AcyI 172 GGCGTC G[GA]^CG[CT]C
AflIII 506 ACGTGT A^C[GA][CT]GT
AlwI 61 GGATC GGATC(4/5)
ApaI 625 GGGCCC GGGCC^C
ApoI 724 AAATTC [GA]^AATT[CT]
Asp718I 38 GGTACC G^GTACC
AvaI 168 CTCGGG C^[CT]CG[GA]G
BanII 625 GGGCCC G[GA]GC[CT]^C
BglI 686 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 172 GGCGTC G[GA]^CG[CT]C
BsaWI 395 TCCGGA [AT]^CCGG[AT]
BseAI 395 TCCGGA T^CCGGA
BseRI 523 GAGGAG GAGGAG(10/8)
BsiWI 306 CGTACG C^GTACG
BsmAI 70 GTCTC GTCTC(1/5)
BsmBI 69 CGTCTC CGTCTC(1/5)
BsmI 479 GAATGC GAATGC(1/-1)
BsoBI 168 CTCGGG C^[CT]CG[GA]G
BspEI 395 TCCGGA T^CCGGA
BsrDI 408 CATTGC GCAATG(2/0)
BsrFI 577 GCCGGC [GA]^CCGG[CT]
BstUI 429 CGCG CG^CG
BstXI 233 CCATCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 61 GGATCT [GA]^GATC[CT]
CfoI 575 GCGC GCG^C
Cfr10I 577 GCCGGC [GA]^CCGG[CT]
DraIII 729 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 428 CCGCGG C^C[GA][CT]GG
EcoT14I 48 CCTTGG C^C[AT][AT]GG
HgaI 173 GCGTC GACGC(5/10)
HhaI 575 GCGC GCG^C
Hin1I 172 GGCGTC G[GA]^CG[CT]C
HinP1I 575 GCGC G^CGC
HphI 613 GGTGA GGTGA(8/7)
KpnI 38 GGTACC GGTAC^C
KspI 428 CCGCGG CCGC^GG
MaeII 506 ACGT A^CGT
MaeIII 614 GTGAC ^GT[ACGT]AC
MflI 61 GGATCT [GA]^GATC[CT]
MroI 395 TCCGGA T^CCGGA
MvnI 429 CGCG CG^CG
NaeI 577 GCCGGC GCC^GGC
NgoMIV 577 GCCGGC G^CCGGC
NspI 566 GCATGT [GA]CATG^[CT]
PflMI 282 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 428 CCGCGG CCGC^GG
SgrAI 576 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 256 CTTGAG C^T[CT][GA]AG
StyI 48 CCTTGG C^C[AT][AT]GG
TfiI 370 GAATC G^A[AT]TC
Tsp45I 614 GTGAC ^GT[GC]AC
Van91I 282 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 61 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 79 AGCT AG^CT
539 AGCT
BalI 2 TGGCCA TGG^CCA
684 TGGCCA
BanI 38 GGTACC G^G[CT][GA]CC
311 GGCACC
BmyI 420 GTGCCC G[AGT]GC[ACT]^C
625 GGGCCC
Bsp1286I 420 GTGCCC G[AGT]GC[ACT]^C
625 GGGCCC
BsrI 141 ACTGG ACTGG(1/-1)
758 CCAGT
BstAPI 189 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
566 GCATGTTCTGC
DdeI 521 CTGAG C^T[ACGT]AG
731 CTGAG
DpnII 62 GATC ^GATC
633 GATC
EaeI 2 TGGCCA [CT]^GGCC[GA]
684 TGGCCA
HapII 396 CCGG C^CGG
578 CCGG
HpaII 396 CCGG C^CGG
578 CCGG
MboI 62 GATC ^GATC
633 GATC
MluNI 2 TGGCCA TGG^CCA
684 TGGCCA
MscI 2 TGGCCA TGG^CCA
684 TGGCCA
MspA1I 428 CCGCGG C[AC]G^C[GT]G
550 CCGCTG
MspI 396 CCGG C^CGG
578 CCGG
NdeII 62 GATC ^GATC
633 GATC
Sau3AI 62 GATC ^GATC
633 GATC
SfaNI 183 GCATC GCATC(5/9)
401 GCATC
Tsp509I 158 AATT ^AATT
725 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI BbvI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cac8I
Cfr13I DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HinfI ItaI
MnlI MvaI MwoI NlaIII NlaIV
PleI PstI RsaI Sau96I ScrFI
SfcI TseI TspRI