################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII BclI BfrI BglII BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 127 GACGTC GACGT^C Acc65I 974 GGTACC G^GTACC AcsI 1507 GAATTC [GA]^AATT[CT] Aor51HI 58 AGCGCT AGC^GCT ApoI 1507 GAATTC [GA]^AATT[CT] Asp718I 974 GGTACC G^GTACC AvrII 969 CCTAGG C^CTAGG BalI 521 TGGCCA TGG^CCA BamHI 651 GGATCC G^GATCC BbeI 1933 GGCGCC GGCGC^C BbrPI 865 CACGTG CAC^GTG BbsI 556 GTCTTC GAAGAC(2/6) BciVI 1108 GGATAC GTATCC(6/5) BfaI 970 CTAG C^TAG BlnI 969 CCTAGG C^CTAGG BlpI 1683 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1683 GCTGAGC GC^T[ACGT]AGC BpuAI 556 GTCTTC GAAGAC(2/6) BsaAI 865 CACGTG [CT]AC^GT[GA] BsaI 1369 GAGACC GGTCTC(1/5) BsrFI 10 GCCGGC [GA]^CCGG[CT] BstEII 429 GGTGACC G^GT[ACGT]ACC BstPI 429 GGTGACC G^GT[ACGT]ACC CelII 1683 GCTGAGC GC^T[ACGT]AGC Cfr10I 10 GCCGGC [GA]^CCGG[CT] DraIII 1756 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 58 AGCGCT AGC^GCT EcoO65I 429 GGTGACC G^GT[ACGT]ACC EcoRI 1507 GAATTC G^AATTC EcoRV 1204 GATATC GAT^ATC EcoT14I 969 CCTAGG C^C[AT][AT]GG HgaI 497 GCGTC GACGC(5/10) KasI 1933 GGCGCC G^GCGCC KpnI 974 GGTACC GGTAC^C KspI 471 CCGCGG CCGC^GG MaeI 970 CTAG C^TAG MluNI 521 TGGCCA TGG^CCA MscI 521 TGGCCA TGG^CCA MslI 1617 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 10 GCCGGC GCC^GGC NarI 1933 GGCGCC GG^CGCC NgoMIV 10 GCCGGC G^CCGGC NspI 1223 GCATGT [GA]CATG^[CT] PmaCI 865 CACGTG CAC^GTG PmlI 865 CACGTG CAC^GTG SacI 1336 GAGCTC GAGCT^C SacII 471 CCGCGG CCGC^GG SexAI 419 ACCTGGT A^CC[AT]GGT SfaNI 1306 GATGC GCATC(5/9) SfoI 1933 GGCGCC GGC^GCC SmlI 1654 CTCAAG C^T[CT][GA]AG StyI 969 CCTAGG C^C[AT][AT]GG Tsp509I 1508 AATT ^AATT ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 127 GACGTC G[GA]^CG[CT]C 1933 GGCGCC AfaI 45 GTAC GT^AC 975 GTAC AocI 724 CCTGAGG CC^T[ACGT]AGG 1407 CCTGAGG AspHI 1003 GAGCAC G[AT]GC[AT]^C 1336 GAGCTC BglI 335 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 863 GCCACGTGGGC BsaHI 127 GACGTC G[GA]^CG[CT]C 1933 GGCGCC BsaWI 911 ACCGGA [AT]^CCGG[AT] 1478 TCCGGT BsiHKAI 1003 GAGCAC G[AT]GC[AT]^C 1336 GAGCTC BsmAI 80 GAGAC GTCTC(1/5) 1369 GAGAC BssSI 312 CACGAG CACGAG(-5/-1) 1339 CTCGTG Bsu36I 724 CCTGAGG CC^T[ACGT]AGG 1407 CCTGAGG DrdI 121 GACAATGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 262 GACTGGGCTGTC EarI 181 CTCTTC CTCTTC(1/4) 1357 GAAGAG Eco81I 724 CCTGAGG CC^T[ACGT]AGG 1407 CCTGAGG EcoNI 885 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1911 CCTGCCCGAGG HaeII 58 AGCGCT [GA]GCGC^[CT] 1933 GGCGCC Hin1I 127 GACGTC G[GA]^CG[CT]C 1933 GGCGCC Ksp632I 181 CTCTTC CTCTTC(1/4) 1357 GAAGAG PpuMI 893 AGGTCCC [GA]G^G[AT]CC[CT] 1170 GGGTCCC RsaI 45 GTAC GT^AC 975 GTAC SmaI 1182 CCCGGG CCC^GGG 1844 CCCGGG StuI 402 AGGCCT AGG^CCT 953 AGGCCT XcmI 400 CCAGGCCTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1595 CCATCCTGGACATGG XmaCI 1182 CCCGGG C^CCGGG 1844 CCCGGG XmaI 1182 CCCGGG C^CCGGG 1844 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AluI AlwI ApaI AvaI AvaII BanI BanII BbvCI BbvI BcgI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfcI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII