Restriction map for
pFN21AE1854
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 226 GACGTC GACGT^C
AccII 18 CGCG CG^CG
Alw44I 787 GTGCAC G^TGCAC
ApaI 275 GGGCCC GGGCC^C
ApaLI 787 GTGCAC G^TGCAC
AviII 158 TGCGCA TGC^GCA
BbeI 501 GGCGCC GGCGC^C
BbrPI 355 CACGTG CAC^GTG
BbvCI 76 GCTGAGG CCTCAGC(-5/-2)
BcgI 281 GCAAGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 652 CTAG C^TAG
BglI 793 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 935 AGATCT A^GATCT
BsaWI 1049 ACCGGA [AT]^CCGG[AT]
BspLU11I 614 ACATGT A^CATGT
BssSI 133 CACGAG CACGAG(-5/-1)
BstAPI 245 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 18 CGCG CG^CG
DdeI 77 CTGAG C^T[ACGT]AG
DraIII 355 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 266 CCAAGG C^C[AT][AT]GG
FauI 278 CCCGC CCCGC(4/6)
FspI 158 TGCGCA TGC^GCA
HaeII 501 GGCGCC [GA]GCGC^[CT]
KasI 501 GGCGCC G^GCGCC
MaeI 652 CTAG C^TAG
MseI 641 TTAA T^TAA
MvnI 18 CGCG CG^CG
NarI 501 GGCGCC GG^CGCC
PmaCI 355 CACGTG CAC^GTG
PmlI 355 CACGTG CAC^GTG
PpuMI 980 GGGACCT [GA]G^G[AT]CC[CT]
SacI 394 GAGCTC GAGCT^C
SfoI 501 GGCGCC GGC^GCC
SmaI 485 CCCGGG CCC^GGG
SphI 953 GCATGC GCATG^C
StyI 266 CCAAGG C^C[AT][AT]GG
Tru9I 641 TTAA T^TAA
XmaCI 485 CCCGGG C^CCGGG
XmaI 485 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 226 GACGTC G[GA]^CG[CT]C
501 GGCGCC
AfaI 567 GTAC GT^AC
870 GTAC
AflIII 599 ACGTGT A^C[GA][CT]GT
614 ACATGT
AlwI 582 GATCC GGATC(4/5)
293 GGATC
BalI 45 TGGCCA TGG^CCA
635 TGGCCA
BbsI 733 GAAGAC GAAGAC(2/6)
921 GAAGAC
BpuAI 733 GAAGAC GAAGAC(2/6)
921 GAAGAC
BsaAI 355 CACGTG [CT]AC^GT[GA]
784 TACGTG
BsaHI 226 GACGTC G[GA]^CG[CT]C
501 GGCGCC
BstEII 360 GGTGACC G^GT[ACGT]ACC
681 GGTCACC
BstPI 360 GGTGACC G^GT[ACGT]ACC
681 GGTCACC
BstXI 671 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
995 CCATCGACCTGG
BstYI 581 AGATCC [GA]^GATC[CT]
935 AGATCT
DraII 274 GGGGCCC [GA]G^G[ACGT]CC[CT]
980 GGGACCT
EarI 202 CTCTTC CTCTTC(1/4)
448 CTCTTC
EcoNI 201 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
696 CCTCATAAAGG
EcoO109I 274 GGGGCCC [GA]G^G[ACGT]CC[CT]
980 GGGACCT
EcoO65I 360 GGTGACC G^GT[ACGT]ACC
681 GGTCACC
Hin1I 226 GACGTC G[GA]^CG[CT]C
501 GGCGCC
Ksp632I 202 CTCTTC CTCTTC(1/4)
448 CTCTTC
MflI 581 AGATCC [GA]^GATC[CT]
935 AGATCT
MluNI 45 TGGCCA TGG^CCA
635 TGGCCA
MscI 45 TGGCCA TGG^CCA
635 TGGCCA
MslI 30 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
948 CATCGGCATG
MspA1I 676 CAGCTG C[AC]G^C[GT]G
1018 CAGCTG
PvuII 676 CAGCTG CAG^CTG
1018 CAGCTG
RsaI 567 GTAC GT^AC
870 GTAC
XhoII 581 AGATCC [GA]^GATC[CT]
935 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I