Restriction map for
pFN21AB6388
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI AocI ApoI AscI AseI
AsnI AspEI AspI AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI Ksp632I
KspI MamI MfeI MluI MluNI
MscI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 838 GGTACC G^GTACC
AccI 37 GTCTAC GT^[AC][GT]AC
AccIII 856 TCCGGA T^CCGGA
AflIII 662 ACGTGT A^C[GA][CT]GT
Alw44I 598 GTGCAC G^TGCAC
AlwNI 480 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 656 AGCGCT AGC^GCT
ApaI 433 GGGCCC GGGCC^C
ApaLI 598 GTGCAC G^TGCAC
Asp700I 120 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 838 GGTACC G^GTACC
AspHI 598 GTGCAC G[AT]GC[AT]^C
AviII 575 TGCGCA TGC^GCA
BamHI 488 GGATCC G^GATCC
BfaI 613 CTAG C^TAG
BsaAI 661 TACGTG [CT]AC^GT[GA]
BsaWI 856 TCCGGA [AT]^CCGG[AT]
BseAI 856 TCCGGA T^CCGGA
BseRI 289 GAGGAG GAGGAG(10/8)
BsiEI 886 CGGCCG CG[GA][CT]^CG
BsiHKAI 598 GTGCAC G[AT]GC[AT]^C
BsmI 745 GAATGC GAATGC(1/-1)
BspEI 856 TCCGGA T^CCGGA
BspMI 550 ACCTGC ACCTGC(4/8)
BssSI 49 CACGAG CACGAG(-5/-1)
BstAPI 14 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 518 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 488 GGATCC [GA]^GATC[CT]
DsaI 623 CCGTGG C^C[GA][CT]GG
EaeI 886 CGGCCG [CT]^GGCC[GA]
EagI 886 CGGCCG C^GGCCG
EclXI 886 CGGCCG C^GGCCG
Eco47III 656 AGCGCT AGC^GCT
Eco52I 886 CGGCCG C^GGCCG
EcoNI 342 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 575 TGCGCA TGC^GCA
HaeII 656 AGCGCT [GA]GCGC^[CT]
KpnI 838 GGTACC GGTAC^C
MaeI 613 CTAG C^TAG
MaeII 662 ACGT A^CGT
MflI 488 GGATCC [GA]^GATC[CT]
MroI 856 TCCGGA T^CCGGA
MslI 816 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 368 GCCGGC GCC^GGC
NgoMIV 368 GCCGGC G^CCGGC
NspI 578 GCATGT [GA]CATG^[CT]
PflMI 479 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 352 GAGTC GAGTC(4/5)
StuI 257 AGGCCT AGG^CCT
TfiI 762 GATTC G^A[AT]TC
TspRI 438 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 479 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 488 GGATCC [GA]^GATC[CT]
XmnI 120 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 501 GACGCC G[GA]^CG[CT]C
591 GACGCC
AfaI 216 GTAC GT^AC
839 GTAC
AvaI 75 CTCGGG C^[CT]CG[GA]G
402 CTCGGG
BsaHI 501 GACGCC G[GA]^CG[CT]C
591 GACGCC
BsgI 21 CTGCAC GTGCAG(16/14)
223 CTGCAC
BsmAI 794 GAGAC GTCTC(1/5)
140 GTCTC
BsmFI 85 GGGAC GGGAC(10/14)
537 GTCCC
BsoBI 75 CTCGGG C^[CT]CG[GA]G
402 CTCGGG
BsrI 790 ACTGG ACTGG(1/-1)
437 CCAGT
BstEII 546 GGTGACC G^GT[ACGT]ACC
735 GGTCACC
BstF5I 207 CATCC GGATG(2/0)
111 GGATG
BstPI 546 GGTGACC G^GT[ACGT]ACC
735 GGTCACC
DdeI 103 CTGAG C^T[ACGT]AG
688 CTGAG
DpnII 163 GATC ^GATC
489 GATC
EcoO65I 546 GGTGACC G^GT[ACGT]ACC
735 GGTCACC
EcoT14I 392 CCAAGG C^C[AT][AT]GG
512 CCAAGG
FokI 207 CATCC GGATG(9/13)
111 GGATG
Hin1I 501 GACGCC G[GA]^CG[CT]C
591 GACGCC
HinfI 352 GAGTC G^A[ACGT]TC
762 GATTC
MboI 163 GATC ^GATC
489 GATC
NdeII 163 GATC ^GATC
489 GATC
PpuMI 85 GGGACCC [GA]G^G[AT]CC[CT]
444 GGGTCCT
PstI 148 CTGCAG CTGCA^G
826 CTGCAG
RsaI 216 GTAC GT^AC
839 GTAC
Sau3AI 163 GATC ^GATC
489 GATC
SfaNI 112 GATGC GCATC(5/9)
894 GATGC
SfcI 148 CTGCAG C^T[GA][CT]AG
826 CTGCAG
StyI 392 CCAAGG C^C[AT][AT]GG
512 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeIII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV Sau96I ScrFI TaqI
TseI Tsp45I TthHB8I