Restriction map for
pFN21AE1725
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 12 GGCGCC G[GA]^CG[CT]C
AlwI 431 GGATC GGATC(4/5)
AlwNI 682 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 239 AGCGCT AGC^GCT
AvaI 63 CTCGGG C^[CT]CG[GA]G
BbeI 12 GGCGCC GGCGC^C
BbsI 356 GTCTTC GAAGAC(2/6)
BlpI 57 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 57 GCTGAGC GC^T[ACGT]AGC
BpuAI 356 GTCTTC GAAGAC(2/6)
BsaHI 12 GGCGCC G[GA]^CG[CT]C
BsaI 397 GAGACC GGTCTC(1/5)
BsgI 613 CTGCAC GTGCAG(16/14)
BsmAI 397 GAGAC GTCTC(1/5)
BsoBI 63 CTCGGG C^[CT]CG[GA]G
BsrBI 250 GAGCGG CCGCTC(-3/-3)
BstAPI 282 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 245 GGATG GGATG(2/0)
BstXI 496 CCACCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 57 GCTGAGC GC^T[ACGT]AGC
DpnII 432 GATC ^GATC
DrdI 118 GACTTCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 596 TGGCCG [CT]^GGCC[GA]
Eco47III 239 AGCGCT AGC^GCT
EcoNI 387 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 502 CCATGG C^C[AT][AT]GG
FokI 245 GGATG GGATG(9/13)
HgaI 627 GACGC GACGC(5/10)
Hin1I 12 GGCGCC G[GA]^CG[CT]C
KasI 12 GGCGCC G^GCGCC
MboI 432 GATC ^GATC
MboII 357 TCTTC GAAGA(8/7)
MslI 497 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 12 GGCGCC GG^CGCC
NcoI 502 CCATGG C^CATGG
NdeII 432 GATC ^GATC
NlaIII 503 CATG CATG^
Sau3AI 432 GATC ^GATC
SfaNI 279 GATGC GCATC(5/9)
SfoI 12 GGCGCC GGC^GCC
SmlI 256 CTCAAG C^T[CT][GA]AG
StyI 502 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 298 CGCG CG^CG
629 CGCG
BcnI 452 CCCGG CC^[GC]GG
512 CCCGG
BstUI 298 CGCG CG^CG
629 CGCG
DdeI 58 CTGAG C^T[ACGT]AG
70 CTCAG
HapII 453 CCGG C^CGG
513 CCGG
HpaII 453 CCGG C^CGG
513 CCGG
HphI 173 TCACC GGTGA(8/7)
323 TCACC
MaeIII 172 GTCAC ^GT[ACGT]AC
322 GTCAC
MspI 453 CCGG C^CGG
513 CCGG
MvnI 298 CGCG CG^CG
629 CGCG
NciI 452 CCCGG CC^[GC]GG
512 CCCGG
PpuMI 76 GGGACCC [GA]G^G[AT]CC[CT]
683 AGGACCT
PvuII 351 CAGCTG CAG^CTG
409 CAGCTG
SacI 60 GAGCTC GAGCT^C
202 GAGCTC
SbfI 189 CCTGCAGG CCTGCA^GG
390 CCTGCAGG
Sse8387I 189 CCTGCAGG CCTGCA^GG
390 CCTGCAGG
Tsp45I 172 GTCAC ^GT[GC]AC
322 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BanII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DraII DsaI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HhaI HinP1I HinfI ItaI
MnlI MspA1I MvaI MwoI NlaIV
PleI PstI Sau96I ScrFI SfcI
TseI TspRI