Restriction map for
pFN21AE2074
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp700I AspEI BalI BbeI BbrPI
BclI BfrI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
Eam1105I Eco81I EcoO65I EcoRV FbaI
FseI HincII HindII HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NheI NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1018 GACGTC GACGT^C
Acc65I 1898 GGTACC G^GTACC
AcsI 61 GAATTC [GA]^AATT[CT]
AcyI 1018 GACGTC G[GA]^CG[CT]C
AflIII 519 ACATGT A^C[GA][CT]GT
AlwNI 1716 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 371 GGGCCC GGGCC^C
ApoI 61 GAATTC [GA]^AATT[CT]
Asp718I 1898 GGTACC G^GTACC
AspI 448 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2163 CCCGAG C^[CT]CG[GA]G
AviII 1154 TGCGCA TGC^GCA
AvrII 1800 CCTAGG C^CTAGG
BamHI 1983 GGATCC G^GATCC
BciVI 402 GGATAC GTATCC(6/5)
BfaI 1801 CTAG C^TAG
BglII 710 AGATCT A^GATCT
BlnI 1800 CCTAGG C^CTAGG
BsaHI 1018 GACGTC G[GA]^CG[CT]C
BsgI 2094 CTGCAC GTGCAG(16/14)
BsoBI 2163 CCCGAG C^[CT]CG[GA]G
BspLU11I 519 ACATGT A^CATGT
BspMI 406 ACCTGC ACCTGC(4/8)
BssHII 1822 GCGCGC G^CGCGC
BstAPI 287 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 88 TTCGAA TT^CGAA
DraI 6 TTTAAA TTT^AAA
DraIII 500 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 987 CGGCCG C^GGCCG
EclXI 987 CGGCCG C^GGCCG
Eco52I 987 CGGCCG C^GGCCG
EcoNI 2193 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 61 GAATTC G^AATTC
EcoT22I 868 ATGCAT ATGCA^T
FspI 1154 TGCGCA TGC^GCA
Hin1I 1018 GACGTC G[GA]^CG[CT]C
HindIII 1207 AAGCTT A^AGCTT
KpnI 1898 GGTACC GGTAC^C
MaeI 1801 CTAG C^TAG
MseI 7 TTAA T^TAA
NsiI 868 ATGCAT ATGCA^T
NspI 519 ACATGT [GA]CATG^[CT]
NspV 88 TTCGAA TT^CGAA
PflFI 448 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 220 AGGACCC [GA]G^G[AT]CC[CT]
PstI 139 CTGCAG CTGCA^G
SacI 394 GAGCTC GAGCT^C
SapI 84 GCTCTTC GCTCTTC(1/4)
SexAI 475 ACCTGGT A^CC[AT]GGT
SfuI 88 TTCGAA TT^CGAA
SmlI 808 CTCAAG C^T[CT][GA]AG
StuI 1844 AGGCCT AGG^CCT
Tru9I 7 TTAA T^TAA
Tth111I 448 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 1984 GATCC GGATC(4/5)
1983 GGATC
Aor51HI 380 AGCGCT AGC^GCT
2018 AGCGCT
BcgI 1878 CGAGGCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2086 CGAGCGTCCTGC
BglI 787 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1382 GCCGAATCGGC
BsiEI 432 CGACCG CG[GA][CT]^CG
987 CGGCCG
BstYI 710 AGATCT [GA]^GATC[CT]
1983 GGATCC
Eco47III 380 AGCGCT AGC^GCT
2018 AGCGCT
FauI 1959 CCCGC CCCGC(4/6)
924 GCGGG
HgaI 448 GACGC GACGC(5/10)
2089 GCGTC
MflI 710 AGATCT [GA]^GATC[CT]
1983 GGATCC
NaeI 985 GCCGGC GCC^GGC
1733 GCCGGC
NgoMIV 985 GCCGGC G^CCGGC
1733 GCCGGC
Tsp509I 62 AATT ^AATT
1120 AATT
XcmI 473 CCACCTGGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2183 CCACCAGCTCCCTGG
XhoII 710 AGATCT [GA]^GATC[CT]
1983 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
ApaLI AspHI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I