Restriction map for
pFN21AE5022
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HindIII HpaI
KpnI MamI MfeI MflI MluI
MroI MseI MunI NcoI NdeI
NotI NruI NsiI NspV PacI
PflFI PmeI PshBI Psp1406I RcaI
RsrII SacI SbfI ScaI SfaNI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 435 GACGTC GACGT^C
AccI 1030 GTCGAC GT^[AC][GT]AC
AfaI 738 GTAC GT^AC
AflIII 387 ACACGT A^C[GA][CT]GT
AlwNI 703 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 523 GTGCTC G[AT]GC[AT]^C
AviII 455 TGCGCA TGC^GCA
BbsI 595 GTCTTC GAAGAC(2/6)
BbvCI 27 CCTCAGC CCTCAGC(-5/-2)
BpmI 424 CTGGAG CTGGAG(16/14)
BpuAI 595 GTCTTC GAAGAC(2/6)
BsiHKAI 523 GTGCTC G[AT]GC[AT]^C
BspMI 662 ACCTGC ACCTGC(4/8)
BssSI 802 CTCGTG CACGAG(-5/-1)
DraIII 939 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 479 CGGCCG C^GGCCG
EclXI 479 CGGCCG C^GGCCG
Eco52I 479 CGGCCG C^GGCCG
EcoNI 678 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 455 TGCGCA TGC^GCA
HincII 1030 GTCGAC GT[CT]^[GA]AC
HindII 1030 GTCGAC GT[CT]^[GA]AC
KspI 144 CCGCGG CCGC^GG
NheI 1261 GCTAGC G^CTAGC
NspI 238 ACATGC [GA]CATG^[CT]
PaeR7I 907 CTCGAG C^TCGAG
PflMI 756 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 835 GACTC GAGTC(4/5)
PpuMI 1061 AGGTCCC [GA]G^G[AT]CC[CT]
PvuI 789 CGATCG CGAT^CG
PvuII 703 CAGCTG CAG^CTG
RsaI 738 GTAC GT^AC
SacII 144 CCGCGG CCGC^GG
SalI 1030 GTCGAC G^TCGAC
SexAI 677 ACCTGGT A^CC[AT]GGT
SgrAI 116 CACCGGTG C[GA]^CCGG[CT]G
SmaI 85 CCCGGG CCC^GGG
TfiI 43 GATTC G^A[AT]TC
Van91I 756 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 907 CTCGAG C^TCGAG
XmaCI 85 CCCGGG C^CCGGG
XmaI 85 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
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AgeI 117 ACCGGT A^CCGGT
1074 ACCGGT
ApaI 14 GGGCCC GGGCC^C
82 GGGCCC
AvaII 750 GGACC G^G[AT]CC
1062 GGTCC
BanII 14 GGGCCC G[GA]GC[CT]^C
82 GGGCCC
BbrPI 388 CACGTG CAC^GTG
691 CACGTG
BsaAI 388 CACGTG [CT]AC^GT[GA]
691 CACGTG
BsaWI 117 ACCGGT [AT]^CCGG[AT]
1074 ACCGGT
BseRI 73 GAGGAG GAGGAG(10/8)
469 GAGGAG
BsgI 340 CTGCAC GTGCAG(16/14)
121 GTGCAG
BsmBI 427 GAGACG CGTCTC(1/5)
586 GAGACG
BsrDI 330 CATTGC GCAATG(2/0)
510 CATTGC
BssHII 209 GCGCGC G^CGCGC
211 GCGCGC
BstXI 236 CCACATGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
395 CCAGCTTCCTGG
DpnII 790 GATC ^GATC
831 GATC
DrdI 429 GACGCAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
529 GACGCCTTCGTC
HinfI 43 GATTC G^A[ACGT]TC
835 GACTC
MboI 790 GATC ^GATC
831 GATC
NdeII 790 GATC ^GATC
831 GATC
PinAI 117 ACCGGT A^CCGGT
1074 ACCGGT
PmaCI 388 CACGTG CAC^GTG
691 CACGTG
PmlI 388 CACGTG CAC^GTG
691 CACGTG
PshAI 588 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1071 GACACCGGTC
SapI 273 GCTCTTC GCTCTTC(1/4)
306 GCTCTTC
Sau3AI 790 GATC ^GATC
831 GATC
SfiI 480 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
555 GGCCAAGATGGCC
SmlI 907 CTCGAG C^T[CT][GA]AG
1129 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BalI BanI BbeI BbvI BcnI
BfaI BglI BmyI BsaHI BsaJI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIII NlaIV PstI Sau96I
ScrFI SfcI SfoI TaqI TseI
Tsp45I TspRI TthHB8I