Restriction map for
pFN21AE9505
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII ClaI
DpnII DraI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MroI MseI MunI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI SacI SacII SalI Sau3AI
SbfI ScaI SfaNI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 426 GTCTAC GT^[AC][GT]AC
AccII 103 CGCG CG^CG
AcyI 76 GGCGCC G[GA]^CG[CT]C
AocI 930 CCTGAGG CC^T[ACGT]AGG
AspI 988 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 409 CTCGGG C^[CT]CG[GA]G
AvaII 51 GGACC G^G[AT]CC
BbeI 76 GGCGCC GGCGC^C
BpmI 579 CTCCAG CTGGAG(16/14)
BsaHI 76 GGCGCC G[GA]^CG[CT]C
BsaI 993 GGTCTC GGTCTC(1/5)
BsaWI 608 TCCGGT [AT]^CCGG[AT]
BseRI 576 CTCCTC GAGGAG(10/8)
BsiEI 694 CGGCCG CG[GA][CT]^CG
BsmFI 939 GTCCC GGGAC(10/14)
BsmI 591 GAATGC GAATGC(1/-1)
BsoBI 409 CTCGGG C^[CT]CG[GA]G
BsrBI 112 CCGCTC CCGCTC(-3/-3)
BstUI 103 CGCG CG^CG
Bsu36I 930 CCTGAGG CC^T[ACGT]AGG
CpoI 50 CGGACCG CG^G[AT]CCG
EagI 694 CGGCCG C^GGCCG
EarI 868 CTCTTC CTCTTC(1/4)
EclXI 694 CGGCCG C^GGCCG
Eco52I 694 CGGCCG C^GGCCG
Eco81I 930 CCTGAGG CC^T[ACGT]AGG
EcoT14I 161 CCATGG C^C[AT][AT]GG
Hin1I 76 GGCGCC G[GA]^CG[CT]C
KasI 76 GGCGCC G^GCGCC
Ksp632I 868 CTCTTC CTCTTC(1/4)
MslI 711 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 103 CGCG CG^CG
NarI 76 GGCGCC GG^CGCC
NcoI 161 CCATGG C^CATGG
PflFI 988 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 937 GAGTC GAGTC(4/5)
PstI 550 CTGCAG CTGCA^G
RsrII 50 CGGACCG CG^G[AT]CCG
SapI 867 GCTCTTC GCTCTTC(1/4)
SfcI 550 CTGCAG C^T[GA][CT]AG
SfoI 76 GGCGCC GGC^GCC
StyI 161 CCATGG C^C[AT][AT]GG
TaqI 92 TCGA T^CGA
TfiI 10 GATTC G^A[AT]TC
Tth111I 988 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 92 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 532 GAGCAC G[AT]GC[AT]^C
763 GTGCTC
BcnI 151 CCGGG CC^[GC]GG
545 CCCGG
BsiHKAI 532 GAGCAC G[AT]GC[AT]^C
763 GTGCTC
BsmAI 255 GTCTC GTCTC(1/5)
994 GTCTC
BspMI 749 ACCTGC ACCTGC(4/8)
453 GCAGGT
BsrDI 279 CATTGC GCAATG(2/0)
155 GCAATG
BstXI 131 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
710 CCATTGTGGTGG
DdeI 382 CTCAG C^T[ACGT]AG
931 CTGAG
HaeII 76 GGCGCC [GA]GCGC^[CT]
324 GGCGCT
HgaI 686 GACGC GACGC(5/10)
362 GCGTC
HinfI 10 GATTC G^A[ACGT]TC
937 GAGTC
HphI 597 GGTGA GGTGA(8/7)
494 TCACC
MboII 134 TCTTC GAAGA(8/7)
869 TCTTC
NaeI 518 GCCGGC GCC^GGC
692 GCCGGC
NciI 151 CCGGG CC^[GC]GG
545 CCCGG
NgoMIV 518 GCCGGC G^CCGGC
692 GCCGGC
PflMI 747 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCATGACCTGG
SexAI 211 ACCTGGT A^CC[AT]GGT
989 ACCTGGT
Van91I 747 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCATGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI ApaI BalI BanI
BanII BbvI BglI BmyI BsaJI
BsgI BsiYI BslI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeIII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NlaIII NlaIV PvuII Sau96I ScrFI
TseI Tsp45I TspRI XcmI