##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     MaeI        MaeIII      MamI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         193  CGCG                 CG^CG
AcsI            4  AAATTT               [GA]^AATT[CT]
AluI          233  AGCT                 AG^CT
AlwNI         118  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI            4  AAATTT               [GA]^AATT[CT]
BbvCI          91  CCTCAGC              CCTCAGC(-5/-2)
BglI          145  GCCCCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmAI         279  GAGAC                GTCTC(1/5)
Bsp1407I      326  TGTACA               T^GTACA
BsrBI         271  GAGCGG               CCGCTC(-3/-3)
BsrGI         326  TGTACA               T^GTACA
BstF5I        242  GGATG                GGATG(2/0)
BstUI         193  CGCG                 CG^CG
BstXI          78  CCAGATTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          143  GCGC                 GCG^C
DdeI           92  CTCAG                C^T[ACGT]AG
DpnII         222  GATC                 ^GATC
DraIII         60  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          192  CCGCGG               C^C[GA][CT]GG
EcoNI         353  CCTTACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          242  GGATG                GGATG(9/13)
HaeII         142  AGCGCC               [GA]GCGC^[CT]
HhaI          143  GCGC                 GCG^C
HinP1I        143  GCGC                 G^CGC
HphI          310  TCACC                GGTGA(8/7)
KspI          192  CCGCGG               CCGC^GG
MaeII          64  ACGT                 A^CGT
MboI          222  GATC                 ^GATC
MslI           29  CATGTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          193  CGCG                 CG^CG
NdeII         222  GATC                 ^GATC
NspI           28  GCATGT               [GA]CATG^[CT]
PflMI          59  CCACGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI          63  GACGTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         232  CAGCTG               CAG^CTG
SacII         192  CCGCGG               CCGC^GG
Sau3AI        222  GATC                 ^GATC
SspBI         326  TGTACA               T^GTACA
Tsp509I         5  AATT                 ^AATT
TspRI         208  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I         59  CCACGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AfaI          267  GTAC                 GT^AC
              327  GTAC                
ApaI          212  GGGCCC               GGGCC^C
              227  GGGCCC              
BanI           42  GGTGCC               G^G[CT][GA]CC
              299  GGCACC              
BpmI          124  CTGGAG               CTGGAG(16/14)
              315  CTGGAG              
BseRI         199  GAGGAG               GAGGAG(10/8)
              286  GAGGAG              
Cac8I         108  GCTGGC               GC[ACGT]^[ACGT]GC
              160  GCGGGC              
FauI          160  GCGGG                CCCGC(4/6)
              194  GCGGG               
PpuMI          68  GGGTCCC              [GA]G^G[AT]CC[CT]
              320  GGGTCCT             
RsaI          267  GTAC                 GT^AC
              327  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AvaI        AvaII       BanII       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BssKI       BstNI       
Cfr13I      DraII       EcoO109I    EcoRII      Fnu4HI      
HaeIII      HapII       HinfI       HpaII       ItaI        
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PleI        Sau96I      
ScrFI       SmaI        TseI        XmaCI       XmaI