Restriction map for
pFN21AB7802
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
MaeI MamI MfeI MluI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI SacI
SalI ScaI SexAI SfuI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tru9I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccIII 112 TCCGGA T^CCGGA
AcsI 472 AAATTC [GA]^AATT[CT]
AflIII 863 ACACGT A^C[GA][CT]GT
Alw44I 1608 GTGCAC G^TGCAC
AocI 791 CCTCAGG CC^T[ACGT]AGG
ApaLI 1608 GTGCAC G^TGCAC
ApoI 472 AAATTC [GA]^AATT[CT]
BbvCI 267 CCTCAGC CCTCAGC(-5/-2)
BcgI 539 GCAGCGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 112 TCCGGA T^CCGGA
BspEI 112 TCCGGA T^CCGGA
BspMI 1664 ACCTGC ACCTGC(4/8)
BsrBI 1164 GAGCGG CCGCTC(-3/-3)
BssHII 160 GCGCGC G^CGCGC
BssSI 1041 CACGAG CACGAG(-5/-1)
Bsu36I 791 CCTCAGG CC^T[ACGT]AGG
CpoI 114 CGGACCG CG^G[AT]CCG
DraIII 1186 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 791 CCTCAGG CC^T[ACGT]AGG
HindIII 208 AAGCTT A^AGCTT
HinfI 962 GAGTC G^A[ACGT]TC
MroI 112 TCCGGA T^CCGGA
PleI 962 GAGTC GAGTC(4/5)
PshAI 1075 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 114 CGGACCG CG^G[AT]CCG
SapI 1170 GAAGAGC GCTCTTC(1/4)
SbfI 123 CCTGCAGG CCTGCA^GG
SfiI 364 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1589 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 123 CCTGCAGG CCTGCA^GG
Tsp509I 473 AATT ^AATT
XcmI 1218 CCAAGCCGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 798 GTAC GT^AC
1656 GTAC
Aor51HI 381 AGCGCT AGC^GCT
731 AGCGCT
BlpI 886 GCTGAGC GC^T[ACGT]AGC
1212 GCTCAGC
Bpu1102I 886 GCTGAGC GC^T[ACGT]AGC
1212 GCTCAGC
BsaWI 112 TCCGGA [AT]^CCGG[AT]
1518 TCCGGT
BsgI 778 CTGCAC GTGCAG(16/14)
1570 CTGCAC
BsmBI 636 CGTCTC CGTCTC(1/5)
922 CGTCTC
BsrFI 293 GCCGGT [GA]^CCGG[CT]
1590 GCCGGT
BstAPI 861 GCACACGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1185 GCACCATGTGC
CelII 886 GCTGAGC GC^T[ACGT]AGC
1212 GCTCAGC
Cfr10I 293 GCCGGT [GA]^CCGG[CT]
1590 GCCGGT
Eco47III 381 AGCGCT AGC^GCT
731 AGCGCT
NcoI 900 CCATGG C^CATGG
1528 CCATGG
PflMI 834 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
895 CCACTCCATGG
PpuMI 1502 GGGTCCT [GA]G^G[AT]CC[CT]
1550 GGGACCC
RsaI 798 GTAC GT^AC
1656 GTAC
StuI 788 AGGCCT AGG^CCT
1700 AGGCCT
Van91I 834 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
895 CCACTCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI Ksp632I
KspI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PmaCI PmlI PstI PvuII SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI