Restriction map for
pFN21AE3003
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# NO CUT ENZYMES ( 197 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DpnII DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HapII HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
MaeI MaeII MamI MboI MboII
MfeI MflI MluI MroI MseI
MspI MunI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 150 GACGCC G[GA]^CG[CT]C
AfaI 157 GTAC GT^AC
Asp700I 171 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 14 TGGCCA TGG^CCA
BcgI 120 CGAGACGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 58 CTGGAG CTGGAG(16/14)
BsaHI 150 GACGCC G[GA]^CG[CT]C
BsiEI 72 CGACCG CG[GA][CT]^CG
BsmAI 121 GAGAC GTCTC(1/5)
BsmBI 121 GAGACG CGTCTC(1/5)
BsrI 154 CCAGT ACTGG(1/-1)
BssKI 108 CCTGG ^CC[ACGT]GG
BstNI 108 CCTGG CC^[AT]GG
BstXI 5 CCAACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 189 GCGC GCG^C
Cfr13I 30 GGCCC G^G[ACGT]CC
DsaI 164 CCGCGG C^C[GA][CT]GG
EaeI 14 TGGCCA [CT]^GGCC[GA]
EcoRII 108 CCTGG ^CC[AT]GG
HaeII 188 AGCGCC [GA]GCGC^[CT]
HhaI 189 GCGC GCG^C
Hin1I 150 GACGCC G[GA]^CG[CT]C
HinP1I 189 GCGC G^CGC
KspI 164 CCGCGG CCGC^GG
MaeIII 148 GTGAC ^GT[ACGT]AC
MluNI 14 TGGCCA TGG^CCA
MscI 14 TGGCCA TGG^CCA
MslI 78 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 108 CCTGG CC^[AT]GG
NspI 126 GCATGC [GA]CATG^[CT]
PvuII 49 CAGCTG CAG^CTG
RsaI 157 GTAC GT^AC
SacII 164 CCGCGG CCGC^GG
Sau96I 30 GGCCC G^G[ACGT]CC
ScrFI 108 CCTGG CC^[ACGT]GG
SphI 126 GCATGC GCATG^C
TaqI 71 TCGA T^CGA
Tsp45I 148 GTGAC ^GT[GC]AC
TthHB8I 71 TCGA T^CGA
XmnI 171 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AccII 165 CGCG CG^CG
181 CGCG
AluI 50 AGCT AG^CT
56 AGCT
BbvI 94 GCAGC GCAGC(8/12)
97 GCAGC
BsaJI 26 CCGAGG C^C[ACGT][ACGT]GG
164 CCGCGG
BsiYI 33 CCGCAAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
75 CCGCATGAAGG
BslI 33 CCGCAAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
75 CCGCATGAAGG
BstUI 165 CGCG CG^CG
181 CGCG
HaeIII 15 GGCC GG^CC
30 GGCC
HgaI 123 GACGC GACGC(5/10)
150 GACGC
HphI 63 GGTGA GGTGA(8/7)
147 GGTGA
MvnI 165 CGCG CG^CG
181 CGCG
TseI 94 GCAGC G^C[AT]GC
97 GCAGC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AciI Cac8I FauI Fnu4HI ItaI
MnlI MspA1I MwoI NlaIII