Restriction map for
pFN21AE1682
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstPI BstUI
BstXI BstYI CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MroI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 524 GTAC GT^AC
AlwI 408 GGATC GGATC(4/5)
AocI 480 CCTGAGG CC^T[ACGT]AGG
AspHI 496 GTGCTC G[AT]GC[AT]^C
AvaII 485 GGTCC G^G[AT]CC
BalI 568 TGGCCA TGG^CCA
BglI 430 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 533 CTCCAG CTGGAG(16/14)
BsiHKAI 496 GTGCTC G[AT]GC[AT]^C
BsiYI 219 CCAGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 219 CCAGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 67 GAGAC GTCTC(1/5)
BsmFI 3 GTCCC GGGAC(10/14)
BssSI 213 CTCGTG CACGAG(-5/-1)
Bst1107I 313 GTATAC GTA^TAC
BstAPI 191 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 313 GTATAC GTA^TAC
Bsu36I 480 CCTGAGG CC^T[ACGT]AGG
CfoI 137 GCGC GCG^C
DpnII 409 GATC ^GATC
DraII 119 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 324 CCGTGG C^C[GA][CT]GG
EaeI 568 TGGCCA [CT]^GGCC[GA]
Eco81I 480 CCTGAGG CC^T[ACGT]AGG
EcoO109I 119 AGGCCCT [GA]G^G[ACGT]CC[CT]
HhaI 137 GCGC GCG^C
HinP1I 137 GCGC G^CGC
HinfI 50 GACTC G^A[ACGT]TC
MaeII 275 ACGT A^CGT
MaeIII 356 GTCAC ^GT[ACGT]AC
MboI 409 GATC ^GATC
MluNI 568 TGGCCA TGG^CCA
MscI 568 TGGCCA TGG^CCA
MspA1I 195 CAGCTG C[AC]G^C[GT]G
NdeII 409 GATC ^GATC
PleI 50 GACTC GAGTC(4/5)
PstI 87 CTGCAG CTGCA^G
PvuII 195 CAGCTG CAG^CTG
RsaI 524 GTAC GT^AC
Sau3AI 409 GATC ^GATC
SfcI 87 CTGCAG C^T[GA][CT]AG
TaqI 48 TCGA T^CGA
Tsp45I 356 GTCAC ^GT[GC]AC
Tsp509I 565 AATT ^AATT
TspRI 260 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 48 TCGA T^CGA
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# TWO CUT ENZYMES ( 7 enzymes) #
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AccI 313 GTATAC GT^[AC][GT]AC
449 GTCTAC
AlwNI 142 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
192 CAGCAGCTG
BanI 343 GGTGCC G^G[CT][GA]CC
414 GGTGCC
BsgI 238 GTGCAG GTGCAG(16/14)
517 GTGCAG
BsrI 261 ACTGG ACTGG(1/-1)
453 ACTGG
MslI 128 CATCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
508 CACCGGGATG
SfaNI 127 GCATC GCATC(5/9)
383 GCATC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI Bsp1286I
BsrBI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII ItaI
MboII MnlI MspI MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TseI