Restriction map for
pFN21AE2647
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BfrI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BseAI BseRI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI Ksp632I MamI MfeI MluI
MroI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tsp509I TspRI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AlwI 114 GATCC GGATC(4/5)
AocI 337 CCTGAGG CC^T[ACGT]AGG
ApaI 759 GGGCCC GGGCC^C
BbeI 448 GGCGCC GGCGC^C
BbsI 604 GAAGAC GAAGAC(2/6)
BclI 431 TGATCA T^GATCA
BfaI 250 CTAG C^TAG
BglII 1322 AGATCT A^GATCT
BpuAI 604 GAAGAC GAAGAC(2/6)
BsaI 181 GAGACC GGTCTC(1/5)
BsaWI 184 ACCGGA [AT]^CCGG[AT]
BsiWI 689 CGTACG C^GTACG
BsmAI 181 GAGAC GTCTC(1/5)
BsmI 276 GAATGC GAATGC(1/-1)
BstAPI 617 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1322 AGATCT [GA]^GATC[CT]
Bsu36I 337 CCTGAGG CC^T[ACGT]AGG
Eco81I 337 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1214 CCAAGG C^C[AT][AT]GG
FbaI 431 TGATCA T^GATCA
HincII 346 GTCAAC GT[CT]^[GA]AC
HindII 346 GTCAAC GT[CT]^[GA]AC
HinfI 19 GATTC G^A[ACGT]TC
KasI 448 GGCGCC G^GCGCC
MaeI 250 CTAG C^TAG
MboII 604 GAAGA GAAGA(8/7)
MflI 1322 AGATCT [GA]^GATC[CT]
MseI 848 TTAA T^TAA
NarI 448 GGCGCC GG^CGCC
NlaIII 485 CATG CATG^
NotI 721 GCGGCCGC GC^GGCCGC
PflMI 1464 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1060 GGGTCCT [GA]G^G[AT]CC[CT]
SbfI 522 CCTGCAGG CCTGCA^GG
SexAI 1247 ACCTGGT A^CC[AT]GGT
SfoI 448 GGCGCC GGC^GCC
Sse8387I 522 CCTGCAGG CCTGCA^GG
StuI 269 AGGCCT AGG^CCT
StyI 1214 CCAAGG C^C[AT][AT]GG
TaqI 38 TCGA T^CGA
TfiI 19 GATTC G^A[AT]TC
Tru9I 848 TTAA T^TAA
TthHB8I 38 TCGA T^CGA
Van91I 1464 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1322 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 448 GGCGCC G[GA]^CG[CT]C
919 GGCGTC
Alw44I 283 GTGCAC G^TGCAC
565 GTGCAC
AlwNI 618 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1297 CAGGAGCTG
ApaLI 283 GTGCAC G^TGCAC
565 GTGCAC
AspHI 283 GTGCAC G[AT]GC[AT]^C
565 GTGCAC
AvaII 306 GGTCC G^G[AT]CC
1061 GGTCC
BalI 662 TGGCCA TGG^CCA
830 TGGCCA
BsaHI 448 GGCGCC G[GA]^CG[CT]C
919 GGCGTC
BsiHKAI 283 GTGCAC G[AT]GC[AT]^C
565 GTGCAC
BsmFI 409 GTCCC GGGAC(10/14)
498 GTCCC
BsrBI 1403 CCGCTC CCGCTC(-3/-3)
238 GAGCGG
BsrFI 679 ACCGGC [GA]^CCGG[CT]
1361 ACCGGC
BstEII 1368 GGTCACC G^GT[ACGT]ACC
1428 GGTCACC
BstPI 1368 GGTCACC G^GT[ACGT]ACC
1428 GGTCACC
Cfr10I 679 ACCGGC [GA]^CCGG[CT]
1361 ACCGGC
EcoNI 249 CCTAGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1263 CCTGGGGAAGG
EcoO65I 1368 GGTCACC G^GT[ACGT]ACC
1428 GGTCACC
HgaI 730 GCGTC GACGC(5/10)
920 GCGTC
Hin1I 448 GGCGCC G[GA]^CG[CT]C
919 GGCGTC
MaeII 647 ACGT A^CGT
1282 ACGT
MluNI 662 TGGCCA TGG^CCA
830 TGGCCA
MscI 662 TGGCCA TGG^CCA
830 TGGCCA
XcmI 332 CCAAGCCTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1214 CCAAGGAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanI BanII BbvI BcnI BglI
BmyI BsaJI BsgI BsiEI BsiYI
BslI BsoBI Bsp1286I BspMI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI KspI MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
TseI Tsp45I