##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BsrI        
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DpnII       DraI        
DrdI        Eam1105I    EarI        Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        HphI        KpnI        Ksp632I     
MaeIII      MamI        MboI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NaeI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        Tru9I       Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI          391  GTCTAC               GT^[AC][GT]AC
AfaI          117  GTAC                 GT^AC
AluI          113  AGCT                 AG^CT
Aor51HI        83  AGCGCT               AGC^GCT
ApaI          132  GGGCCC               GGGCC^C
AvaI          591  CCCGGG               C^[CT]CG[GA]G
BanII         132  GGGCCC               G[GA]GC[CT]^C
BbeI          300  GGCGCC               GGCGC^C
BfaI          428  CTAG                 C^TAG
BsaI          184  GAGACC               GGTCTC(1/5)
BsoBI         591  CCCGGG               C^[CT]CG[GA]G
BspMI         236  ACCTGC               ACCTGC(4/8)
BsrBI         384  GAGCGG               CCGCTC(-3/-3)
BssSI         247  CTCGTG               CACGAG(-5/-1)
BstF5I        555  CATCC                GGATG(2/0)
DdeI          581  CTGAG                C^T[ACGT]AG
Eco47III       83  AGCGCT               AGC^GCT
FokI          555  CATCC                GGATG(9/13)
KasI          300  GGCGCC               G^GCGCC
MaeI          428  CTAG                 C^TAG
NarI          300  GGCGCC               GG^CGCC
PflMI         363  CCACGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          190  GACTC                GAGTC(4/5)
RsaI          117  GTAC                 GT^AC
SfoI          300  GGCGCC               GGC^GCC
SmaI          591  CCCGGG               CCC^GGG
StuI          275  AGGCCT               AGG^CCT
Van91I        363  CCACGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         591  CCCGGG               C^CCGGG
XmaI          591  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcyI            3  GGCGTC               G[GA]^CG[CT]C
              300  GGCGCC              
BanI           51  GGTGCC               G^G[CT][GA]CC
              300  GGCGCC              
BcgI           81  CGAGCGCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              230  CGAGGGACCTGC        
BmyI          132  GGGCCC               G[AGT]GC[ACT]^C
              282  GGGCAC              
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
              300  GGCGCC              
BsgI          253  GTGCAG               GTGCAG(16/14)
              401  GTGCAG              
BsmBI          43  GAGACG               CGTCTC(1/5)
               70  GAGACG              
Bsp1286I      132  GGGCCC               G[AGT]GC[ACT]^C
              282  GGGCAC              
BssHII        313  GCGCGC               G^CGCGC
              487  GCGCGC              
DraIII        364  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
              472  CACAGCGTG           
HaeII          83  AGCGCT               [GA]GCGC^[CT]
              300  GGCGCC              
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
              300  GGCGCC              
MaeII         368  ACGT                 A^CGT
              413  ACGT                
NotI          143  GCGGCCGC             GC^GGCCGC
              373  GCGGCCGC            
TfiI           76  GATTC                G^A[AT]TC
              262  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AccII       AciI        AvaII       BbvI        BcnI        
BglI        BsaJI       BseRI       BsiEI       BsiYI       
BslI        BsmAI       BsmFI       BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DraII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoO109I    EcoRII      FauI        Fnu4HI      HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       ItaI        KspI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIV       PpuMI       SacII       Sau96I      ScrFI       
TseI