Restriction map for
pFN21AE2282
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DpnII DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MboI MboII MfeI
MflI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AflIII 308 ACGTGT A^C[GA][CT]GT
ApaI 48 GGGCCC GGGCC^C
AspHI 31 GTGCTC G[AT]GC[AT]^C
AvaI 44 CCCGGG C^[CT]CG[GA]G
BglI 50 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 8 CTCCAG CTGGAG(16/14)
BsaAI 307 TACGTG [CT]AC^GT[GA]
BsiHKAI 31 GTGCTC G[AT]GC[AT]^C
BsmFI 131 GTCCC GGGAC(10/14)
BsoBI 44 CCCGGG C^[CT]CG[GA]G
BsrI 10 CCAGT ACTGG(1/-1)
BstF5I 291 GGATG GGATG(2/0)
BstXI 281 CCACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 349 GCGC GCG^C
EcoT14I 215 CCATGG C^C[AT][AT]GG
FauI 168 GCGGG CCCGC(4/6)
FokI 291 GGATG GGATG(9/13)
HaeII 348 GGCGCT [GA]GCGC^[CT]
HgaI 86 GACGC GACGC(5/10)
HhaI 349 GCGC GCG^C
HinP1I 349 GCGC G^CGC
HphI 231 GGTGA GGTGA(8/7)
NcoI 215 CCATGG C^CATGG
NlaIII 216 CATG CATG^
PleI 324 GACTC GAGTC(4/5)
PstI 319 CTGCAG CTGCA^G
PvuII 316 CAGCTG CAG^CTG
SfcI 319 CTGCAG C^T[GA][CT]AG
SmaI 44 CCCGGG CCC^GGG
StyI 215 CCATGG C^C[AT][AT]GG
TspRI 11 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 212 TGGCCA TGG^CCA
296 TGGCCA
BanII 48 GGGCCC G[GA]GC[CT]^C
92 GAGCCC
BcnI 44 CCCGG CC^[GC]GG
45 CCGGG
BssSI 173 CACGAG CACGAG(-5/-1)
247 CACGAG
Cfr13I 48 GGGCC G^G[ACGT]CC
49 GGCCC
DraII 47 GGGGCCC [GA]G^G[ACGT]CC[CT]
48 GGGCCCT
DsaI 66 CCGTGG C^C[GA][CT]GG
215 CCATGG
EcoO109I 47 GGGGCCC [GA]G^G[ACGT]CC[CT]
48 GGGCCCT
HapII 36 CCGG C^CGG
45 CCGG
HpaII 36 CCGG C^CGG
45 CCGG
MaeII 241 ACGT A^CGT
308 ACGT
MluNI 212 TGGCCA TGG^CCA
296 TGGCCA
MscI 212 TGGCCA TGG^CCA
296 TGGCCA
MspA1I 166 CAGCGG C[AC]G^C[GT]G
316 CAGCTG
MspI 36 CCGG C^CGG
45 CCGG
NciI 44 CCCGG CC^[GC]GG
45 CCGGG
Sau96I 48 GGGCC G^G[ACGT]CC
49 GGCCC
SmlI 99 CTTGAG C^T[CT][GA]AG
140 CTCAAG
TfiI 145 GAATC G^A[AT]TC
154 GAATC
Tsp45I 312 GTCAC ^GT[GC]AC
365 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I EaeI EcoRII Fnu4HI HaeIII
HinfI ItaI MaeIII MnlI MslI
MvaI MwoI NlaIV ScrFI TseI