Restriction map for
pFN21AE1666
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BseAI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
HincII HindII HpaI KpnI Ksp632I
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MspA1I MunI NaeI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 273 GACGTC GACGT^C
Aor51HI 155 AGCGCT AGC^GCT
AviII 335 TGCGCA TGC^GCA
BbsI 454 GTCTTC GAAGAC(2/6)
BcgI 56 CGACCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 93 CCGGG CC^[GC]GG
BlpI 45 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
BpuAI 454 GTCTTC GAAGAC(2/6)
BsaWI 82 TCCGGT [AT]^CCGG[AT]
BseRI 532 GAGGAG GAGGAG(10/8)
BsgI 86 GTGCAG GTGCAG(16/14)
BsiEI 449 CGGCCG CG[GA][CT]^CG
BsiWI 201 CGTACG C^GTACG
BsmFI 276 GTCCC GGGAC(10/14)
BstXI 473 CCACCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 45 GCTGAGC GC^T[ACGT]AGC
DdeI 46 CTGAG C^T[ACGT]AG
EagI 449 CGGCCG C^GGCCG
EclXI 449 CGGCCG C^GGCCG
Eco47III 155 AGCGCT AGC^GCT
Eco52I 449 CGGCCG C^GGCCG
EcoT14I 341 CCTTGG C^C[AT][AT]GG
FspI 335 TGCGCA TGC^GCA
HindIII 352 AAGCTT A^AGCTT
NciI 93 CCGGG CC^[GC]GG
PleI 346 GAGTC GAGTC(4/5)
PstI 538 CTGCAG CTGCA^G
ScaI 215 AGTACT AGT^ACT
SfcI 538 CTGCAG C^T[GA][CT]AG
StyI 341 CCTTGG C^C[AT][AT]GG
TfiI 75 GATTC G^A[AT]TC
Tsp45I 271 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 115 CGCG CG^CG
117 CGCG
AspHI 135 GAGCAC G[AT]GC[AT]^C
358 GTGCTC
AvaII 15 GGACC G^G[AT]CC
147 GGACC
BanI 110 GGCGCC G^G[CT][GA]CC
313 GGCGCC
BanII 378 GAGCCC G[GA]GC[CT]^C
444 GGGCTC
BbeI 110 GGCGCC GGCGC^C
313 GGCGCC
BsiHKAI 135 GAGCAC G[AT]GC[AT]^C
358 GTGCTC
BsrBI 48 GAGCGG CCGCTC(-3/-3)
106 GAGCGG
BstF5I 123 CATCC GGATG(2/0)
255 CATCC
BstUI 115 CGCG CG^CG
117 CGCG
EaeI 366 TGGCCG [CT]^GGCC[GA]
449 CGGCCG
FauI 170 CCCGC CCCGC(4/6)
442 GCGGG
FokI 123 CATCC GGATG(9/13)
255 CATCC
HinfI 75 GATTC G^A[ACGT]TC
346 GAGTC
HphI 270 GGTGA GGTGA(8/7)
375 GGTGA
KasI 110 GGCGCC G^GCGCC
313 GGCGCC
MaeII 209 ACGT A^CGT
274 ACGT
MaeIII 271 GTGAC ^GT[ACGT]AC
320 GTTAC
MboII 425 TCTTC GAAGA(8/7)
455 TCTTC
MvnI 115 CGCG CG^CG
117 CGCG
NarI 110 GGCGCC GG^CGCC
313 GGCGCC
SfoI 110 GGCGCC GGC^GCC
313 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcyI AfaI AluI BbvI
BmyI BpmI BsaHI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I EcoRII Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI MnlI MslI MspI
MvaI MwoI NlaIV RsaI Sau96I
ScrFI SfaNI TseI