Restriction map for
pFN21AE6640
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbvCI BcgI BciVI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tsp509I
TspRI Tth111I TthHB8I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 70 GTCTAC GT^[AC][GT]AC
AcyI 145 GACGCC G[GA]^CG[CT]C
AfaI 266 GTAC GT^AC
AlwI 446 GGATC GGATC(4/5)
AlwNI 91 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
BbrPI 222 CACGTG CAC^GTG
BbsI 142 GAAGAC GAAGAC(2/6)
BclI 272 TGATCA T^GATCA
BpuAI 142 GAAGAC GAAGAC(2/6)
BsaHI 145 GACGCC G[GA]^CG[CT]C
BseRI 49 CTCCTC GAGGAG(10/8)
DdeI 233 CTGAG C^T[ACGT]AG
DraIII 192 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 52 CTCTTC CTCTTC(1/4)
FauI 162 CCCGC CCCGC(4/6)
FbaI 272 TGATCA T^GATCA
Hin1I 145 GACGCC G[GA]^CG[CT]C
Ksp632I 52 CTCTTC CTCTTC(1/4)
MaeIII 190 GTCAC ^GT[ACGT]AC
MslI 306 CATATACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 664 CAGCTG C[AC]G^C[GT]G
PflMI 630 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 222 CACGTG CAC^GTG
PmlI 222 CACGTG CAC^GTG
PvuII 664 CAGCTG CAG^CTG
RsaI 266 GTAC GT^AC
SexAI 193 ACCTGGT A^CC[AT]GGT
StuI 567 AGGCCT AGG^CCT
TfiI 428 GAATC G^A[AT]TC
Tsp45I 190 GTCAC ^GT[GC]AC
Van91I 630 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 389 CCAGGCCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 221 ACACGT A^C[GA][CT]GT
311 ACGTGT
ApaI 78 GGGCCC GGGCC^C
243 GGGCCC
BanII 78 GGGCCC G[GA]GC[CT]^C
243 GGGCCC
BpmI 616 CTCCAG CTGGAG(16/14)
667 CTGGAG
BsaAI 222 CACGTG [CT]AC^GT[GA]
310 TACGTG
BsmFI 478 GGGAC GGGAC(10/14)
112 GTCCC
DpnII 273 GATC ^GATC
447 GATC
DsaI 210 CCATGG C^C[GA][CT]GG
297 CCATGG
EcoT14I 210 CCATGG C^C[AT][AT]GG
297 CCATGG
HphI 129 GGTGA GGTGA(8/7)
191 TCACC
MboI 273 GATC ^GATC
447 GATC
NcoI 210 CCATGG C^CATGG
297 CCATGG
NdeII 273 GATC ^GATC
447 GATC
PleI 17 GAGTC GAGTC(4/5)
337 GAGTC
PpuMI 110 AGGTCCC [GA]G^G[AT]CC[CT]
254 AGGACCC
Sau3AI 273 GATC ^GATC
447 GATC
SmaI 246 CCCGGG CCC^GGG
608 CCCGGG
StyI 210 CCATGG C^C[AT][AT]GG
297 CCATGG
XmaCI 246 CCCGGG C^CCGGG
608 CCCGGG
XmaI 246 CCCGGG C^CCGGG
608 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanI BbvI BcnI BglI BmyI
BsaJI BsiYI BslI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MaeII MboII MnlI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI