Restriction map for
pFN21AE2609
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI Sse8387I
SspBI StuI StyI SwaI Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 369 ACACGT A^C[GA][CT]GT
AluI 741 AGCT AG^CT
AlwI 327 GATCC GGATC(4/5)
ApaI 277 GGGCCC GGGCC^C
AspI 682 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 667 GGTGCC G^G[CT][GA]CC
BfaI 157 CTAG C^TAG
BsaAI 418 TACGTA [CT]AC^GT[GA]
BsaWI 468 TCCGGT [AT]^CCGG[AT]
BsiEI 291 CGGTCG CG[GA][CT]^CG
BsmI 35 GCATTC GAATGC(1/-1)
BspHI 761 TCATGA T^CATGA
BspMI 86 ACCTGC ACCTGC(4/8)
BstF5I 782 GGATG GGATG(2/0)
CfoI 614 GCGC GCG^C
DpnII 327 GATC ^GATC
FauI 280 CCCGC CCCGC(4/6)
FokI 782 GGATG GGATG(9/13)
HaeII 613 AGCGCC [GA]GCGC^[CT]
HaeIII 278 GGCC GG^CC
HapII 469 CCGG C^CGG
HgaI 349 GACGC GACGC(5/10)
HhaI 614 GCGC GCG^C
HinP1I 614 GCGC G^CGC
HincII 503 GTCAAC GT[CT]^[GA]AC
HindII 503 GTCAAC GT[CT]^[GA]AC
HpaII 469 CCGG C^CGG
MaeI 157 CTAG C^TAG
MboI 327 GATC ^GATC
MboII 397 GAAGA GAAGA(8/7)
MspI 469 CCGG C^CGG
NdeII 327 GATC ^GATC
NspI 219 GCATGC [GA]CATG^[CT]
PflFI 682 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 747 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 740 CAGCTG CAG^CTG
RcaI 761 TCATGA T^CATGA
Sau3AI 327 GATC ^GATC
ScaI 771 AGTACT AGT^ACT
SfaNI 758 GCATC GCATC(5/9)
SnaBI 418 TACGTA TAC^GTA
SphI 219 GCATGC GCATG^C
SspI 109 AATATT AAT^ATT
Tth111I 682 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 177 CCAGAATCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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BanII 49 GGGCTC G[GA]GC[CT]^C
277 GGGCCC
BbvI 573 GCTGC GCAGC(8/12)
576 GCTGC
BsmAI 298 GAGAC GTCTC(1/5)
511 GAGAC
MseI 73 TTAA T^TAA
496 TTAA
MspA1I 740 CAGCTG C[AC]G^C[GT]G
749 CCGCTG
NlaIII 220 CATG CATG^
762 CATG
TaqI 231 TCGA T^CGA
294 TCGA
TfiI 180 GAATC G^A[AT]TC
356 GAATC
Tru9I 73 TTAA T^TAA
496 TTAA
TseI 573 GCTGC G^C[AT]GC
576 GCTGC
Tsp509I 234 AATT ^AATT
413 AATT
TthHB8I 231 TCGA T^CGA
294 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AvaII BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI HinfI
HphI ItaI MaeII MaeIII MnlI
MvaI MwoI NlaIV PleI PpuMI
RsaI Sau96I ScrFI Tsp45I TspRI