Restriction map for
pFN21AE4201
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1144 GTCTAC GT^[AC][GT]AC
AcyI 1967 GGCGCC G[GA]^CG[CT]C
AfaI 92 GTAC GT^AC
Alw44I 1282 GTGCAC G^TGCAC
ApaLI 1282 GTGCAC G^TGCAC
Asp700I 55 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1362 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1967 GGCGCC GGCGC^C
BbrPI 1354 CACGTG CAC^GTG
BglI 1822 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1182 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1967 GGCGCC G[GA]^CG[CT]C
BsaWI 178 ACCGGA [AT]^CCGG[AT]
BsmBI 1567 CGTCTC CGTCTC(1/5)
BspDI 350 ATCGAT AT^CGAT
ClaI 350 ATCGAT AT^CGAT
EcoNI 1752 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1967 GGCGCC G[GA]^CG[CT]C
KasI 1967 GGCGCC G^GCGCC
KspI 1383 CCGCGG CCGC^GG
MamI 1182 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1430 TTAA T^TAA
NaeI 1827 GCCGGC GCC^GGC
NarI 1967 GGCGCC GG^CGCC
NcoI 1742 CCATGG C^CATGG
NgoMIV 1827 GCCGGC G^CCGGC
PflFI 1362 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1354 CACGTG CAC^GTG
PmlI 1354 CACGTG CAC^GTG
RsaI 92 GTAC GT^AC
SacI 1099 GAGCTC GAGCT^C
SacII 1383 CCGCGG CCGC^GG
SbfI 1765 CCTGCAGG CCTGCA^GG
SfoI 1967 GGCGCC GGC^GCC
Sse8387I 1765 CCTGCAGG CCTGCA^GG
StuI 872 AGGCCT AGG^CCT
TfiI 86 GATTC G^A[AT]TC
Tru9I 1430 TTAA T^TAA
Tsp509I 610 AATT ^AATT
Tth111I 1362 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 55 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 993 CGCG CG^CG
1384 CGCG
AlwNI 464 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2002 CAGGAACTG
AocI 557 CCTCAGG CC^T[ACGT]AGG
568 CCTGAGG
AviII 236 TGCGCA TGC^GCA
1067 TGCGCA
BamHI 116 GGATCC G^GATCC
252 GGATCC
BbsI 140 GTCTTC GAAGAC(2/6)
211 GTCTTC
BpuAI 140 GTCTTC GAAGAC(2/6)
211 GTCTTC
BsaAI 1042 TACGTG [CT]AC^GT[GA]
1354 CACGTG
BsaI 175 GAGACC GGTCTC(1/5)
2068 GGTCTC
BsmI 527 GAATGC GAATGC(1/-1)
936 GAATGC
BsrBI 147 GAGCGG CCGCTC(-3/-3)
1504 GAGCGG
BstEII 382 GGTTACC G^GT[ACGT]ACC
1128 GGTCACC
BstPI 382 GGTTACC G^GT[ACGT]ACC
1128 GGTCACC
BstUI 993 CGCG CG^CG
1384 CGCG
Bsu36I 557 CCTCAGG CC^T[ACGT]AGG
568 CCTGAGG
DrdI 202 GACCCTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1084 GACCTCCTTGTC
Eco81I 557 CCTCAGG CC^T[ACGT]AGG
568 CCTGAGG
EcoO65I 382 GGTTACC G^GT[ACGT]ACC
1128 GGTCACC
EcoT14I 501 CCTTGG C^C[AT][AT]GG
1742 CCATGG
FspI 236 TGCGCA TGC^GCA
1067 TGCGCA
MvnI 993 CGCG CG^CG
1384 CGCG
PvuII 676 CAGCTG CAG^CTG
1801 CAGCTG
SapI 1263 GCTCTTC GCTCTTC(1/4)
1557 GCTCTTC
SmaI 49 CCCGGG CCC^GGG
734 CCCGGG
StyI 501 CCTTGG C^C[AT][AT]GG
1742 CCATGG
XmaCI 49 CCCGGG C^CCGGG
734 CCCGGG
XmaI 49 CCCGGG C^CCGGG
734 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII