Restriction map for
pFN21AE1692
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# NO CUT ENZYMES ( 155 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 334 GACGTC GACGT^C
AccII 533 CGCG CG^CG
AlwNI 56 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 234 CCTGAGG CC^T[ACGT]AGG
ApaI 415 GGGCCC GGGCC^C
BanII 415 GGGCCC G[GA]GC[CT]^C
BbsI 214 GTCTTC GAAGAC(2/6)
BbvCI 400 CCTCAGC CCTCAGC(-5/-2)
BciVI 392 GTATCC GTATCC(6/5)
BlpI 277 GCTGAGC GC^T[ACGT]AGC
BmyI 415 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 277 GCTGAGC GC^T[ACGT]AGC
BpuAI 214 GTCTTC GAAGAC(2/6)
BsaI 536 GAGACC GGTCTC(1/5)
BseRI 526 CTCCTC GAGGAG(10/8)
BsmAI 536 GAGAC GTCTC(1/5)
Bsp1286I 415 GGGCCC G[AGT]GC[ACT]^C
BspMI 110 GCAGGT ACCTGC(4/8)
BsrFI 452 ACCGGC [GA]^CCGG[CT]
BstF5I 369 CATCC GGATG(2/0)
BstUI 533 CGCG CG^CG
Bsu36I 234 CCTGAGG CC^T[ACGT]AGG
CelII 277 GCTGAGC GC^T[ACGT]AGC
CfoI 258 GCGC GCG^C
Cfr10I 452 ACCGGC [GA]^CCGG[CT]
Eco81I 234 CCTGAGG CC^T[ACGT]AGG
FokI 369 CATCC GGATG(9/13)
HaeII 257 AGCGCC [GA]GCGC^[CT]
HhaI 258 GCGC GCG^C
HinP1I 258 GCGC G^CGC
HincII 579 GTCAAC GT[CT]^[GA]AC
HindII 579 GTCAAC GT[CT]^[GA]AC
MvnI 533 CGCG CG^CG
NheI 138 GCTAGC G^CTAGC
PstI 5 CTGCAG CTGCA^G
SfaNI 499 GCATC GCATC(5/9)
SfcI 5 CTGCAG C^T[GA][CT]AG
SmlI 181 CTCAAG C^T[CT][GA]AG
StuI 386 AGGCCT AGG^CCT
Tsp509I 226 AATT ^AATT
TspRI 442 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 158 CCAGGGACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 163 GACGCC G[GA]^CG[CT]C
334 GACGTC
AvaI 220 CCCGGG C^[CT]CG[GA]G
425 CCCGGG
BglI 101 GCCACATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
268 GCCTTGGAGGC
BsaHI 163 GACGCC G[GA]^CG[CT]C
334 GACGTC
BsgI 361 CTGCAC GTGCAG(16/14)
448 CTGCAC
BsoBI 220 CCCGGG C^[CT]CG[GA]G
425 CCCGGG
DsaI 17 CCGTGG C^C[GA][CT]GG
50 CCGTGG
EaeI 47 TGGCCG [CT]^GGCC[GA]
551 TGGCCG
EarI 517 CTCTTC CTCTTC(1/4)
172 GAAGAG
EcoT14I 167 CCTTGG C^C[AT][AT]GG
269 CCTTGG
HgaI 163 GACGC GACGC(5/10)
291 GACGC
Hin1I 163 GACGCC G[GA]^CG[CT]C
334 GACGTC
Ksp632I 517 CTCTTC CTCTTC(1/4)
172 GAAGAG
MaeII 335 ACGT A^CGT
577 ACGT
MspA1I 311 CCGCTG C[AC]G^C[GT]G
482 CCGCTG
PpuMI 230 GGGACCT [GA]G^G[AT]CC[CT]
299 AGGTCCT
SmaI 220 CCCGGG CCC^GGG
425 CCCGGG
StyI 167 CCTTGG C^C[AT][AT]GG
269 CCTTGG
XmaCI 220 CCCGGG C^CCGGG
425 CCCGGG
XmaI 220 CCCGGG C^CCGGG
425 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BfaI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboII
MnlI MspI MvaI MwoI NciI
NlaIV PleI Sau96I ScrFI TseI
Tsp45I