Restriction map for
pFN21AE1684
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BclI
BfaI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I ClaI CpoI
DpnII DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI MaeI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StyI
SwaI TfiI Tth111I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AocI 243 CCTGAGG CC^T[ACGT]AGG
ApaI 430 GGGCCC GGGCC^C
BbvCI 415 CCTCAGC CCTCAGC(-5/-2)
BciVI 407 GTATCC GTATCC(6/5)
BlpI 286 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 286 GCTGAGC GC^T[ACGT]AGC
BsaI 551 GAGACC GGTCTC(1/5)
BseRI 541 CTCCTC GAGGAG(10/8)
BspMI 255 GCAGGT ACCTGC(4/8)
BsrBI 213 CCGCTC CCGCTC(-3/-3)
BsrFI 467 ACCGGC [GA]^CCGG[CT]
Bsu36I 243 CCTGAGG CC^T[ACGT]AGG
CelII 286 GCTGAGC GC^T[ACGT]AGC
CfoI 267 GCGC GCG^C
Cfr10I 467 ACCGGC [GA]^CCGG[CT]
DrdI 577 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 35 TGGCCG [CT]^GGCC[GA]
Eco81I 243 CCTGAGG CC^T[ACGT]AGG
EcoNI 155 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 266 AGCGCC [GA]GCGC^[CT]
HgaI 300 GACGC GACGC(5/10)
HhaI 267 GCGC GCG^C
HinP1I 267 GCGC G^CGC
KspI 81 CCGCGG CCGC^GG
MseI 158 TTAA T^TAA
MslI 15 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 15 CATG CATG^
NspI 14 GCATGC [GA]CATG^[CT]
PaeR7I 508 CTCGAG C^TCGAG
PflMI 230 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 250 CAGCTG CAG^CTG
SacII 81 CCGCGG CCGC^GG
SmaI 440 CCCGGG CCC^GGG
SmlI 508 CTCGAG C^T[CT][GA]AG
SphI 14 GCATGC GCATG^C
TaqI 509 TCGA T^CGA
Tru9I 158 TTAA T^TAA
Tsp509I 559 AATT ^AATT
TthHB8I 509 TCGA T^CGA
Van91I 230 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 508 CTCGAG C^TCGAG
XmaCI 440 CCCGGG C^CCGGG
XmaI 440 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 82 CGCG CG^CG
548 CGCG
AvaI 440 CCCGGG C^[CT]CG[GA]G
508 CTCGAG
BanII 86 GGGCTC G[GA]GC[CT]^C
430 GGGCCC
BmyI 86 GGGCTC G[AGT]GC[ACT]^C
430 GGGCCC
BsgI 376 CTGCAC GTGCAG(16/14)
463 CTGCAC
BsmAI 551 GAGAC GTCTC(1/5)
135 GTCTC
BsoBI 440 CCCGGG C^[CT]CG[GA]G
508 CTCGAG
Bsp1286I 86 GGGCTC G[AGT]GC[ACT]^C
430 GGGCCC
BstF5I 96 GGATG GGATG(2/0)
348 GGATG
BstUI 82 CGCG CG^CG
548 CGCG
FokI 96 GGATG GGATG(9/13)
348 GGATG
HinfI 184 GAGTC G^A[ACGT]TC
493 GAGTC
HphI 258 GGTGA GGTGA(8/7)
521 TCACC
MaeIII 52 GTGAC ^GT[ACGT]AC
520 GTCAC
MvnI 82 CGCG CG^CG
548 CGCG
PleI 184 GAGTC GAGTC(4/5)
493 GAGTC
PstI 148 CTGCAG CTGCA^G
253 CTGCAG
SfaNI 97 GATGC GCATC(5/9)
172 GATGC
SfcI 148 CTGCAG C^T[GA][CT]AG
253 CTGCAG
StuI 152 AGGCCT AGG^CCT
512 AGGCCT
Tsp45I 52 GTGAC ^GT[GC]AC
520 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DraII DsaI EarI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HpaII ItaI Ksp632I MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIV PpuMI Sau96I ScrFI
TseI TspRI