Restriction map for
pFN21AE3995
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII AvrII
BamHI BbsI BbvCI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssSI Bst1107I BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MflI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI TfiI
Tru9I Tth111I Van91I XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcsI 652 AAATTC [GA]^AATT[CT]
AfaI 603 GTAC GT^AC
AflIII 716 ACGTGT A^C[GA][CT]GT
AhdI 608 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 440 GGGCCC GGGCC^C
ApoI 652 AAATTC [GA]^AATT[CT]
AspEI 608 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 715 CACGTG CAC^GTG
BlpI 111 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC
BsaAI 715 CACGTG [CT]AC^GT[GA]
BsaI 12 GGTCTC GGTCTC(1/5)
BsgI 247 CTGCAC GTGCAG(16/14)
BsiEI 234 CGGCCG CG[GA][CT]^CG
Bsp1407I 602 TGTACA T^GTACA
BspMI 492 GCAGGT ACCTGC(4/8)
BsrFI 232 ACCGGC [GA]^CCGG[CT]
BsrGI 602 TGTACA T^GTACA
BstAPI 347 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 757 TTCGAA TT^CGAA
CelII 111 GCTGAGC GC^T[ACGT]AGC
Cfr10I 232 ACCGGC [GA]^CCGG[CT]
DsaI 222 CCACGG C^C[GA][CT]GG
EagI 234 CGGCCG C^GGCCG
Eam1105I 608 GACTCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 61 GAAGAG CTCTTC(1/4)
EclXI 234 CGGCCG C^GGCCG
Eco52I 234 CGGCCG C^GGCCG
EcoNI 504 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HphI 710 TCACC GGTGA(8/7)
Ksp632I 61 GAAGAG CTCTTC(1/4)
MboII 61 GAAGA GAAGA(8/7)
NspV 757 TTCGAA TT^CGAA
PmaCI 715 CACGTG CAC^GTG
PmlI 715 CACGTG CAC^GTG
PpuMI 35 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 603 GTAC GT^AC
SfiI 358 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 757 TTCGAA TT^CGAA
SmaI 32 CCCGGG CCC^GGG
SspBI 602 TGTACA T^GTACA
TaqI 758 TCGA T^CGA
Tsp509I 653 AATT ^AATT
TthHB8I 758 TCGA T^CGA
XcmI 725 CCAACATCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 32 CCCGGG C^CCGGG
XmaI 32 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 207 GATCC GGATC(4/5)
670 GATCC
AlwNI 190 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
591 CAGCGGCTG
AvaI 32 CCCGGG C^[CT]CG[GA]G
139 CTCGGG
BalI 523 TGGCCA TGG^CCA
792 TGGCCA
BanI 4 GGCGCC G^G[CT][GA]CC
226 GGCGCC
BbeI 4 GGCGCC GGCGC^C
226 GGCGCC
BcgI 131 GCACGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
320 GCAGACCCATCG
BglI 296 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
359 GCCAAGAAGGC
BpmI 15 CTCCAG CTGGAG(16/14)
819 CTCCAG
BsoBI 32 CCCGGG C^[CT]CG[GA]G
139 CTCGGG
BstF5I 675 CATCC GGATG(2/0)
380 GGATG
FokI 675 CATCC GGATG(9/13)
380 GGATG
HinfI 608 GACTC G^A[ACGT]TC
836 GAGTC
KasI 4 GGCGCC G^GCGCC
226 GGCGCC
MluNI 523 TGGCCA TGG^CCA
792 TGGCCA
MscI 523 TGGCCA TGG^CCA
792 TGGCCA
MslI 711 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
729 CATCAAGATG
NarI 4 GGCGCC GG^CGCC
226 GGCGCC
PleI 608 GACTC GAGTC(4/5)
836 GAGTC
PvuII 261 CAGCTG CAG^CTG
474 CAGCTG
SfoI 4 GGCGCC GGC^GCC
226 GGCGCC
StuI 289 AGGCCT AGG^CCT
366 AGGCCT
TspRI 682 CAGTG CA[GC]TG[ACGT][ACGT]^
822 CAGTG
XbaI 624 TCTAGA T^CTAGA
645 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanII BbvI BcnI BfaI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI MaeI MaeII
MaeIII MboI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI
Tsp45I