Restriction map for
pFN21AE0242
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BbvCI BciVI BclI
BfrI BglII BlnI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MslI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AocI 421 CCTGAGG CC^T[ACGT]AGG
BalI 479 TGGCCA TGG^CCA
BamHI 675 GGATCC G^GATCC
BbeI 720 GGCGCC GGCGC^C
BbrPI 1149 CACGTG CAC^GTG
BbsI 367 GAAGAC GAAGAC(2/6)
BcgI 891 CGAGCGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 85 CTAG C^TAG
BglI 802 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 367 GAAGAC GAAGAC(2/6)
BsmBI 694 GAGACG CGTCTC(1/5)
BsrBI 304 CCGCTC CCGCTC(-3/-3)
BsrDI 1173 CATTGC GCAATG(2/0)
BssSI 889 CACGAG CACGAG(-5/-1)
Bsu36I 421 CCTGAGG CC^T[ACGT]AGG
Eco81I 421 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1318 CCATGG C^C[AT][AT]GG
KasI 720 GGCGCC G^GCGCC
MaeI 85 CTAG C^TAG
MluNI 479 TGGCCA TGG^CCA
MscI 479 TGGCCA TGG^CCA
MseI 1310 TTAA T^TAA
MspA1I 1075 CAGCTG C[AC]G^C[GT]G
NarI 720 GGCGCC GG^CGCC
NcoI 1318 CCATGG C^CATGG
PaeR7I 983 CTCGAG C^TCGAG
PmaCI 1149 CACGTG CAC^GTG
PmlI 1149 CACGTG CAC^GTG
PvuII 1075 CAGCTG CAG^CTG
SacI 1099 GAGCTC GAGCT^C
SfoI 720 GGCGCC GGC^GCC
SmaI 1208 CCCGGG CCC^GGG
StyI 1318 CCATGG C^C[AT][AT]GG
TaqI 984 TCGA T^CGA
TfiI 520 GATTC G^A[AT]TC
Tru9I 1310 TTAA T^TAA
TthHB8I 984 TCGA T^CGA
XhoI 983 CTCGAG C^TCGAG
XmaCI 1208 CCCGGG C^CCGGG
XmaI 1208 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 720 GGCGCC G[GA]^CG[CT]C
1015 GACGCC
AfaI 489 GTAC GT^AC
1137 GTAC
ApaI 88 GGGCCC GGGCC^C
461 GGGCCC
AvaI 983 CTCGAG C^[CT]CG[GA]G
1208 CCCGGG
AviII 506 TGCGCA TGC^GCA
900 TGCGCA
BlpI 306 GCTCAGC GC^T[ACGT]AGC
1215 GCTCAGC
Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC
1215 GCTCAGC
BsaAI 475 TACGTG [CT]AC^GT[GA]
1149 CACGTG
BsaHI 720 GGCGCC G[GA]^CG[CT]C
1015 GACGCC
BsaI 184 GAGACC GGTCTC(1/5)
285 GGTCTC
BsaWI 768 ACCGGA [AT]^CCGG[AT]
846 ACCGGA
BsoBI 983 CTCGAG C^[CT]CG[GA]G
1208 CCCGGG
BspMI 8 ACCTGC ACCTGC(4/8)
1301 ACCTGC
BstXI 344 CCAGCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
534 CCACGGGTCTGG
CelII 306 GCTCAGC GC^T[ACGT]AGC
1215 GCTCAGC
EaeI 479 TGGCCA [CT]^GGCC[GA]
551 CGGCCA
FspI 506 TGCGCA TGC^GCA
900 TGCGCA
HgaI 1015 GACGC GACGC(5/10)
1263 GACGC
Hin1I 720 GGCGCC G[GA]^CG[CT]C
1015 GACGCC
MaeIII 1336 GTGAC ^GT[ACGT]AC
1349 GTGAC
NlaIII 250 CATG CATG^
1319 CATG
PflMI 15 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
447 CCAGTCTCTGG
PleI 616 GACTC GAGTC(4/5)
749 GACTC
RsaI 489 GTAC GT^AC
1137 GTAC
SfaNI 509 GCATC GCATC(5/9)
884 GCATC
SmlI 218 CTCAAG C^T[CT][GA]AG
983 CTCGAG
Tsp45I 1336 GTGAC ^GT[GC]AC
1349 GTGAC
TspRI 1246 CAGTG CA[GC]TG[ACGT][ACGT]^
1347 CAGTG
Van91I 15 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
447 CCAGTCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIV PpuMI Sau3AI
Sau96I ScrFI TseI XhoII