Restriction map for
pFN21AE5271
##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BlnI BlpI BmyI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MfeI MluI MroI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp509I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AhdI 622 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 202 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 89 AGCGCT AGC^GCT
AspEI 622 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 406 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 77 TGGCCA TGG^CCA
BbsI 403 GAAGAC GAAGAC(2/6)
BbvI 468 GCTGC GCAGC(8/12)
BglI 136 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 557 AGATCT A^GATCT
BpuAI 403 GAAGAC GAAGAC(2/6)
BsaBI 558 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 328 GAGACC GGTCTC(1/5)
BseRI 645 CTCCTC GAGGAG(10/8)
BsmBI 100 GAGACG CGTCTC(1/5)
BsmFI 317 GGGAC GGGAC(10/14)
BstEII 192 GGTCACC G^GT[ACGT]ACC
BstPI 192 GGTCACC G^GT[ACGT]ACC
BstXI 632 CCAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 90 GCGC GCG^C
DdeI 68 CTGAG C^T[ACGT]AG
DraIII 246 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 188 CCACGG C^C[GA][CT]GG
Eam1105I 622 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 577 GAAGAG CTCTTC(1/4)
Eco47III 89 AGCGCT AGC^GCT
EcoNI 207 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 192 GGTCACC G^GT[ACGT]ACC
EcoT14I 110 CCAAGG C^C[AT][AT]GG
HaeII 89 AGCGCT [GA]GCGC^[CT]
HgaI 102 GACGC GACGC(5/10)
HhaI 90 GCGC GCG^C
HinP1I 90 GCGC G^CGC
Ksp632I 577 GAAGAG CTCTTC(1/4)
MaeII 29 ACGT A^CGT
MaeIII 193 GTCAC ^GT[ACGT]AC
MamI 558 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 77 TGGCCA TGG^CCA
MscI 77 TGGCCA TGG^CCA
MseI 587 TTAA T^TAA
MspA1I 462 CCGCTG C[AC]G^C[GT]G
NlaIV 317 GGGACC GG[ACGT]^[ACGT]CC
PflFI 406 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 429 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 317 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 186 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 567 CTGTAG C^T[GA][CT]AG
SmlI 43 CTCAAG C^T[CT][GA]AG
StuI 344 AGGCCT AGG^CCT
StyI 110 CCAAGG C^C[AT][AT]GG
Tru9I 587 TTAA T^TAA
TseI 468 GCTGC G^C[AT]GC
Tsp45I 193 GTCAC ^GT[GC]AC
TspRI 624 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 406 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 429 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 593 CCAAGAACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 155 AGCT AG^CT
506 AGCT
AlwI 204 GATCC GGATC(4/5)
489 GGATC
AvaI 313 CTCGGG C^[CT]CG[GA]G
484 CTCGGG
AvaII 318 GGACC G^G[AT]CC
375 GGACC
BsmAI 100 GAGAC GTCTC(1/5)
328 GAGAC
BsoBI 313 CTCGGG C^[CT]CG[GA]G
484 CTCGGG
BspMI 320 ACCTGC ACCTGC(4/8)
331 ACCTGC
BsrI 394 ACTGG ACTGG(1/-1)
162 CCAGT
BstF5I 363 GGATG GGATG(2/0)
518 GGATG
DraII 215 AGGCCCT [GA]G^G[ACGT]CC[CT]
317 GGGACCT
EcoO109I 215 AGGCCCT [GA]G^G[ACGT]CC[CT]
317 GGGACCT
Fnu4HI 231 GCCGC GC^[ACGT]GC
468 GCTGC
FokI 363 GGATG GGATG(9/13)
518 GGATG
HinfI 406 GACTC G^A[ACGT]TC
547 GAGTC
ItaI 231 GCCGC GC^[ACGT]GC
468 GCTGC
MwoI 136 GCCAACCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
524 GCAAATACGGC
PleI 406 GACTC GAGTC(4/5)
547 GAGTC
SfaNI 480 GATGC GCATC(5/9)
635 GCATC
TaqI 53 TCGA T^CGA
266 TCGA
TthHB8I 53 TCGA T^CGA
266 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AfaI BpmI BsaJI BsiYI
BslI BssKI BstNI BstYI Cac8I
Cfr13I DpnII EaeI EcoRII HaeIII
HphI MboI MboII MflI MnlI
MvaI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI XhoII