Restriction map for
pFN21AE6601
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HindIII HpaI
HphI KpnI Ksp632I MaeIII MamI
MboII MfeI MflI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SalI SapI SbfI ScaI SfaNI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
XbaI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 427 GACGTC GACGT^C
AviII 338 TGCGCA TGC^GCA
BfaI 679 CTAG C^TAG
BsgI 313 CTGCAC GTGCAG(16/14)
BsrBI 226 GAGCGG CCGCTC(-3/-3)
BsrFI 571 GCCGGC [GA]^CCGG[CT]
BstF5I 642 CATCC GGATG(2/0)
Cfr10I 571 GCCGGC [GA]^CCGG[CT]
DpnII 480 GATC ^GATC
DraIII 64 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 421 GACTTCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 328 CGGCCG C^GGCCG
EclXI 328 CGGCCG C^GGCCG
Eco52I 328 CGGCCG C^GGCCG
EcoNI 105 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 642 CATCC GGATG(9/13)
FspI 338 TGCGCA TGC^GCA
HincII 502 GTCAAC GT[CT]^[GA]AC
HindII 502 GTCAAC GT[CT]^[GA]AC
HinfI 94 GACTC G^A[ACGT]TC
KspI 322 CCGCGG CCGC^GG
MaeI 679 CTAG C^TAG
MboI 480 GATC ^GATC
NaeI 571 GCCGGC GCC^GGC
NdeII 480 GATC ^GATC
NgoMIV 571 GCCGGC G^CCGGC
NotI 327 GCGGCCGC GC^GGCCGC
NspI 275 ACATGC [GA]CATG^[CT]
PaeR7I 406 CTCGAG C^TCGAG
PflMI 60 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 94 GACTC GAGTC(4/5)
PpuMI 458 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 550 GAGCTC GAGCT^C
SacII 322 CCGCGG CCGC^GG
Sau3AI 480 GATC ^GATC
SexAI 65 ACCTGGT A^CC[AT]GGT
SmlI 406 CTCGAG C^T[CT][GA]AG
Van91I 60 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 623 CCAGGAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 406 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 387 GAGCAC G[AT]GC[AT]^C
550 GAGCTC
AvaI 246 CTCGGG C^[CT]CG[GA]G
406 CTCGAG
BanII 34 GGGCTC G[GA]GC[CT]^C
550 GAGCTC
BglI 321 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
529 GCCCGGCAGGC
BlpI 384 GCTGAGC GC^T[ACGT]AGC
699 GCTGAGC
BpmI 289 CTGGAG CTGGAG(16/14)
448 CTGGAG
Bpu1102I 384 GCTGAGC GC^T[ACGT]AGC
699 GCTGAGC
BsiHKAI 387 GAGCAC G[AT]GC[AT]^C
550 GAGCTC
BsoBI 246 CTCGGG C^[CT]CG[GA]G
406 CTCGAG
BstAPI 99 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
111 GCAGGAGCTGC
CelII 384 GCTGAGC GC^T[ACGT]AGC
699 GCTGAGC
DdeI 385 CTGAG C^T[ACGT]AG
700 CTGAG
DsaI 322 CCGCGG C^C[GA][CT]GG
560 CCACGG
HgaI 126 GACGC GACGC(5/10)
281 GACGC
MaeII 428 ACGT A^CGT
506 ACGT
MslI 342 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
489 CATGGAGATG
NlaIII 276 CATG CATG^
489 CATG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaII BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiEI
BsiYI BslI Bsp1286I BspMI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
KasI MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NlaIV
Sau96I ScrFI SfoI TaqI TseI
TthHB8I