Restriction map for
pFN21AB9713
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanII BbrPI BbsI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI Tru9I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 61 GACGTC GACGT^C
AcsI 645 GAATTT [GA]^AATT[CT]
AfaI 1112 GTAC GT^AC
Aor51HI 952 AGCGCT AGC^GCT
ApoI 645 GAATTT [GA]^AATT[CT]
AvaI 286 CCCGAG C^[CT]CG[GA]G
BbvCI 268 GCTGAGG CCTCAGC(-5/-2)
BcgI 662 CGACGGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 864 GGATAC GTATCC(6/5)
BsaWI 797 TCCGGT [AT]^CCGG[AT]
BsgI 279 GTGCAG GTGCAG(16/14)
BsmI 209 GCATTC GAATGC(1/-1)
BsoBI 286 CCCGAG C^[CT]CG[GA]G
BsrBI 308 GAGCGG CCGCTC(-3/-3)
BsrFI 322 GCCGGC [GA]^CCGG[CT]
BssSI 54 CACGAG CACGAG(-5/-1)
Cfr10I 322 GCCGGC [GA]^CCGG[CT]
DraII 881 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 335 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 952 AGCGCT AGC^GCT
EcoO109I 881 GGGTCCC [GA]G^G[ACGT]CC[CT]
HincII 909 GTCAAC GT[CT]^[GA]AC
HindII 909 GTCAAC GT[CT]^[GA]AC
KspI 40 CCGCGG CCGC^GG
MaeIII 466 GTCAC ^GT[ACGT]AC
MslI 561 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 322 GCCGGC GCC^GGC
NgoMIV 322 GCCGGC G^CCGGC
PflMI 93 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 981 GACTC GAGTC(4/5)
PpuMI 881 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 900 CAGCTG CAG^CTG
RsaI 1112 GTAC GT^AC
SacII 40 CCGCGG CCGC^GG
SapI 730 GAAGAGC GCTCTTC(1/4)
SexAI 336 ACCAGGT A^CC[AT]GGT
SphI 123 GCATGC GCATG^C
TfiI 794 GAATC G^A[AT]TC
Tsp45I 466 GTCAC ^GT[GC]AC
Van91I 93 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 223 GATCC GGATC(4/5)
399 GATCC
AlwNI 94 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
238 CAGCTCCTG
AocI 243 CCTGAGG CC^T[ACGT]AGG
511 CCTAAGG
AvaII 882 GGTCC G^G[AT]CC
934 GGACC
BanI 44 GGCGCC G^G[CT][GA]CC
535 GGCGCC
BbeI 44 GGCGCC GGCGC^C
535 GGCGCC
BfaI 424 CTAG C^TAG
917 CTAG
BsaI 1088 GAGACC GGTCTC(1/5)
768 GGTCTC
BspMI 1065 ACCTGC ACCTGC(4/8)
1080 ACCTGC
BsrI 776 ACTGG ACTGG(1/-1)
1109 CCAGT
BstYI 222 AGATCC [GA]^GATC[CT]
398 AGATCC
Bsu36I 243 CCTGAGG CC^T[ACGT]AGG
511 CCTAAGG
Eco81I 243 CCTGAGG CC^T[ACGT]AGG
511 CCTAAGG
HgaI 232 GACGC GACGC(5/10)
961 GACGC
HinfI 794 GAATC G^A[ACGT]TC
981 GACTC
KasI 44 GGCGCC G^GCGCC
535 GGCGCC
MaeI 424 CTAG C^TAG
917 CTAG
MaeII 62 ACGT A^CGT
464 ACGT
MflI 222 AGATCC [GA]^GATC[CT]
398 AGATCC
NarI 44 GGCGCC GG^CGCC
535 GGCGCC
NspI 123 GCATGC [GA]CATG^[CT]
257 ACATGC
PstI 67 CTGCAG CTGCA^G
1021 CTGCAG
SfoI 44 GGCGCC GGC^GCC
535 GGCGCC
StuI 440 AGGCCT AGG^CCT
499 AGGCCT
TaqI 50 TCGA T^CGA
623 TCGA
Tsp509I 133 AATT ^AATT
646 AATT
TspRI 191 CAGTG CA[GC]TG[ACGT][ACGT]^
596 CAGTG
TthHB8I 50 TCGA T^CGA
623 TCGA
XcmI 725 CCAAAGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
996 CCAGCCTCCTCCTGG
XhoII 222 AGATCC [GA]^GATC[CT]
398 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
BalI BbvI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoNI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI TseI