Restriction map for
pFN21AE1618
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr13I
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NarI NciI
NcoI NdeI NheI NlaIII NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcsI 86 AAATTC [GA]^AATT[CT]
AlwNI 121 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 86 AAATTC [GA]^AATT[CT]
BanII 52 GAGCCC G[GA]GC[CT]^C
BbsI 15 GAAGAC GAAGAC(2/6)
BbvI 120 GCAGC GCAGC(8/12)
BciVI 104 GTATCC GTATCC(6/5)
BglII 5 AGATCT A^GATCT
BmyI 52 GAGCCC G[AGT]GC[ACT]^C
BpmI 148 CTGGAG CTGGAG(16/14)
BpuAI 15 GAAGAC GAAGAC(2/6)
BsgI 199 CTGCAC GTGCAG(16/14)
BsiYI 198 CCTGCACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 198 CCTGCACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 365 GTCCC GGGAC(10/14)
Bsp1286I 52 GAGCCC G[AGT]GC[ACT]^C
BspMI 359 ACCTGC ACCTGC(4/8)
BsrFI 136 GCCGGC [GA]^CCGG[CT]
BssKI 204 CCTGG ^CC[ACGT]GG
BssSI 314 CTCGTG CACGAG(-5/-1)
BstNI 204 CCTGG CC^[AT]GG
BstYI 5 AGATCT [GA]^GATC[CT]
CfoI 213 GCGC GCG^C
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
DdeI 168 CTCAG C^T[ACGT]AG
DraIII 202 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 156 TGGCCG [CT]^GGCC[GA]
EcoRII 204 CCTGG ^CC[AT]GG
HaeIII 157 GGCC GG^CC
HapII 137 CCGG C^CGG
HhaI 213 GCGC GCG^C
HinP1I 213 GCGC G^CGC
HincII 322 GTCAAC GT[CT]^[GA]AC
HindII 322 GTCAAC GT[CT]^[GA]AC
HinfI 190 GAGTC G^A[ACGT]TC
HpaII 137 CCGG C^CGG
MflI 5 AGATCT [GA]^GATC[CT]
MslI 310 CACCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 145 CAGCTG C[AC]G^C[GT]G
MspI 137 CCGG C^CGG
MvaI 204 CCTGG CC^[AT]GG
NaeI 136 GCCGGC GCC^GGC
NgoMIV 136 GCCGGC G^CCGGC
PleI 190 GAGTC GAGTC(4/5)
PvuII 145 CAGCTG CAG^CTG
ScrFI 204 CCTGG CC^[ACGT]GG
SexAI 203 ACCTGGT A^CC[AT]GGT
SmlI 43 CTTGAG C^T[CT][GA]AG
TaqI 50 TCGA T^CGA
TseI 120 GCAGC G^C[AT]GC
Tsp509I 87 AATT ^AATT
TthHB8I 50 TCGA T^CGA
XcmI 194 CCACCCTGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 5 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AluI 146 AGCT AG^CT
251 AGCT
BsrI 25 ACTGG ACTGG(1/-1)
56 CCAGT
BstF5I 180 CATCC GGATG(2/0)
153 GGATG
DpnII 6 GATC ^GATC
282 GATC
FokI 180 CATCC GGATG(9/13)
153 GGATG
HgaI 123 GCGTC GACGC(5/10)
363 GCGTC
HphI 38 TCACC GGTGA(8/7)
309 TCACC
MaeIII 59 GTGAC ^GT[ACGT]AC
347 GTCAC
MboI 6 GATC ^GATC
282 GATC
MwoI 287 GCCGCAAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
296 GCTATGCTCGC
NdeII 6 GATC ^GATC
282 GATC
Sau3AI 6 GATC ^GATC
282 GATC
Tsp45I 59 GTGAC ^GT[GC]AC
347 GTCAC
TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^
57 CAGTG
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AciI Cac8I Fnu4HI ItaI MboII
MnlI