Restriction map for
pFN21AE5426
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AlwNI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I EcoNI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HapII Hin1I HincII HindII
HindIII HinfI HpaI HpaII KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 236 CGCG CG^CG
AcsI 31 GAATTC [GA]^AATT[CT]
AhdI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 751 GTGCAC G^TGCAC
Aor51HI 266 AGCGCT AGC^GCT
ApaLI 751 GTGCAC G^TGCAC
ApoI 31 GAATTC [GA]^AATT[CT]
AspEI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 508 GGATCC G^GATCC
BanI 940 GGCACC G^G[CT][GA]CC
BanII 408 GAGCCC G[GA]GC[CT]^C
BpmI 147 CTGGAG CTGGAG(16/14)
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsiEI 360 CGACCG CG[GA][CT]^CG
BsmAI 202 GTCTC GTCTC(1/5)
BsmFI 794 GTCCC GGGAC(10/14)
BspHI 404 TCATGA T^CATGA
BsrI 293 CCAGT ACTGG(1/-1)
BstEII 474 GGTGACC G^GT[ACGT]ACC
BstPI 474 GGTGACC G^GT[ACGT]ACC
BstUI 236 CGCG CG^CG
Eam1105I 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 266 AGCGCT AGC^GCT
EcoO65I 474 GGTGACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
FauI 234 CCCGC CCCGC(4/6)
HaeII 266 AGCGCT [GA]GCGC^[CT]
HgaI 191 GCGTC GACGC(5/10)
MspA1I 581 CAGCTG C[AC]G^C[GT]G
MvnI 236 CGCG CG^CG
NspI 239 GCATGC [GA]CATG^[CT]
PflMI 140 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 581 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SapI 78 GCTCTTC GCTCTTC(1/4)
SfaNI 75 GATGC GCATC(5/9)
SmlI 781 CTCAAG C^T[CT][GA]AG
SphI 239 GCATGC GCATG^C
StuI 710 AGGCCT AGG^CCT
Tsp509I 32 AATT ^AATT
Van91I 140 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 272 CCATCGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 235 CCGC CCGC(-3/-1)
363 CCGC
AluI 316 AGCT AG^CT
582 AGCT
AocI 876 CCTCAGG CC^T[ACGT]AGG
935 CCTCAGG
AspHI 400 GTGCTC G[AT]GC[AT]^C
751 GTGCAC
AvaII 793 GGTCC G^G[AT]CC
911 GGACC
BbvCI 855 CCTCAGC CCTCAGC(-5/-2)
918 CCTCAGC
BseRI 637 CTCCTC GAGGAG(10/8)
646 CTCCTC
BsiHKAI 400 GTGCTC G[AT]GC[AT]^C
751 GTGCAC
BspMI 125 ACCTGC ACCTGC(4/8)
251 ACCTGC
Bsu36I 876 CCTCAGG CC^T[ACGT]AGG
935 CCTCAGG
DraII 792 AGGTCCC [GA]G^G[ACGT]CC[CT]
910 AGGACCT
DsaI 221 CCGTGG C^C[GA][CT]GG
412 CCACGG
Eco81I 876 CCTCAGG CC^T[ACGT]AGG
935 CCTCAGG
EcoO109I 792 AGGTCCC [GA]G^G[ACGT]CC[CT]
910 AGGACCT
MaeII 368 ACGT A^CGT
398 ACGT
MaeIII 475 GTGAC ^GT[ACGT]AC
812 GTGAC
PpuMI 792 AGGTCCC [GA]G^G[AT]CC[CT]
910 AGGACCT
Tsp45I 475 GTGAC ^GT[GC]AC
812 GTGAC
TspRI 113 CACTG CA[GC]TG[ACGT][ACGT]^
620 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AlwI BalI BbvI BglI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HphI ItaI Ksp632I
MboI MboII MflI MluNI MnlI
MscI MslI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TseI XhoII