Restriction map for
pFN21AE4214
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# NO CUT ENZYMES ( 92 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI AspEI
AviII AvrII BamHI BciVI BclI
BfaI BfrI BlnI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstZ17I ClaI CpoI DraI DraIII
Eam1105I EarI Eco47III EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI Ksp632I MaeI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspI PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 297 GGTACC G^GTACC
AflIII 2195 ACGTGT A^C[GA][CT]GT
AgeI 2086 ACCGGT A^CCGGT
Asp700I 21 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 297 GGTACC G^GTACC
AspHI 2176 GAGCTC G[AT]GC[AT]^C
AspI 1137 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1798 TGGCCA TGG^CCA
BbrPI 2029 CACGTG CAC^GTG
BglII 326 AGATCT A^GATCT
BsaAI 2029 CACGTG [CT]AC^GT[GA]
BsaI 820 GGTCTC GGTCTC(1/5)
BsiHKAI 2176 GAGCTC G[AT]GC[AT]^C
BsmBI 1614 CGTCTC CGTCTC(1/5)
BssHII 987 GCGCGC G^CGCGC
BssSI 1712 CTCGTG CACGAG(-5/-1)
BstAPI 1779 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2095 TTCGAA TT^CGAA
BstXI 1742 CCAGTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 782 CGGCCG C^GGCCG
EclXI 782 CGGCCG C^GGCCG
Eco52I 782 CGGCCG C^GGCCG
EcoRV 1399 GATATC GAT^ATC
KpnI 297 GGTACC GGTAC^C
KspI 1911 CCGCGG CCGC^GG
MluNI 1798 TGGCCA TGG^CCA
MscI 1798 TGGCCA TGG^CCA
NcoI 1493 CCATGG C^CATGG
NspV 2095 TTCGAA TT^CGAA
PflFI 1137 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2086 ACCGGT A^CCGGT
PmaCI 2029 CACGTG CAC^GTG
PmlI 2029 CACGTG CAC^GTG
PstI 1789 CTGCAG CTGCA^G
SacI 2176 GAGCTC GAGCT^C
SacII 1911 CCGCGG CCGC^GG
SfcI 1789 CTGCAG C^T[GA][CT]AG
SfuI 2095 TTCGAA TT^CGAA
SmlI 1423 CTCAAG C^T[CT][GA]AG
TfiI 2133 GAATC G^A[AT]TC
Tth111I 1137 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1251 CCAGAAGTGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 21 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 1804 GATCC GGATC(4/5)
2163 GGATC
AocI 346 CCTGAGG CC^T[ACGT]AGG
1111 CCTCAGG
BcgI 573 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2103 CGACTTCGATGC
BsaWI 705 ACCGGA [AT]^CCGG[AT]
2086 ACCGGT
BsiEI 782 CGGCCG CG[GA][CT]^CG
1914 CGGTCG
BsmAI 821 GTCTC GTCTC(1/5)
1615 GTCTC
BstEII 682 GGTTACC G^GT[ACGT]ACC
2082 GGTCACC
BstPI 682 GGTTACC G^GT[ACGT]ACC
2082 GGTCACC
Bsu36I 346 CCTGAGG CC^T[ACGT]AGG
1111 CCTCAGG
DrdI 1298 GACTGTACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2059 GACGAGGAGGTC
Eco81I 346 CCTGAGG CC^T[ACGT]AGG
1111 CCTCAGG
EcoNI 1377 CCTATCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1470 CCTGTATAAGG
EcoO65I 682 GGTTACC G^GT[ACGT]ACC
2082 GGTCACC
MaeII 2030 ACGT A^CGT
2195 ACGT
MslI 501 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1767 CATCGAGGTG
PflMI 388 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1086 CCAACAGCTGG
StuI 1708 AGGCCT AGG^CCT
1734 AGGCCT
Van91I 388 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1086 CCAACAGCTGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwNI ApaI AvaI AvaII BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI SgrAI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII XmaCI XmaI