Restriction map for
pFN21AE5331
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlpI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Asp700I 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 115 GTGCTC G[AT]GC[AT]^C
AvaI 118 CTCGGG C^[CT]CG[GA]G
AvaII 625 GGTCC G^G[AT]CC
AvrII 765 CCTAGG C^CTAGG
BamHI 638 GGATCC G^GATCC
BanII 896 GGGCTC G[GA]GC[CT]^C
BfaI 766 CTAG C^TAG
BlnI 765 CCTAGG C^CTAGG
BsaAI 674 TACGTG [CT]AC^GT[GA]
BsiHKAI 115 GTGCTC G[AT]GC[AT]^C
BsmAI 742 GTCTC GTCTC(1/5)
BsoBI 118 CTCGGG C^[CT]CG[GA]G
Bsp1407I 830 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BsrGI 830 TGTACA T^GTACA
BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 638 GGATCC [GA]^GATC[CT]
EcoRI 462 GAATTC G^AATTC
EcoT14I 765 CCTAGG C^C[AT][AT]GG
HindIII 477 AAGCTT A^AGCTT
MaeI 766 CTAG C^TAG
MaeII 675 ACGT A^CGT
MflI 638 GGATCC [GA]^GATC[CT]
MwoI 346 GCTGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RcaI 404 TCATGA T^CATGA
SexAI 104 ACCTGGT A^CC[AT]GGT
SfcI 867 CTACAG C^T[GA][CT]AG
SspBI 830 TGTACA T^GTACA
StyI 765 CCTAGG C^C[AT][AT]GG
TfiI 123 GAATC G^A[AT]TC
XhoII 638 GGATCC [GA]^GATC[CT]
XmnI 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 31 GAATTT [GA]^AATT[CT]
462 GAATTC
AlwI 639 GATCC GGATC(4/5)
638 GGATC
ApoI 31 GAATTT [GA]^AATT[CT]
462 GAATTC
BmyI 115 GTGCTC G[AGT]GC[ACT]^C
896 GGGCTC
BsaJI 412 CCTCGG C^C[ACGT][ACGT]GG
765 CCTAGG
BsgI 388 CTGCAC GTGCAG(16/14)
504 CTGCAC
BsmFI 45 GGGAC GGGAC(10/14)
626 GTCCC
Bsp1286I 115 GTGCTC G[AGT]GC[ACT]^C
896 GGGCTC
Cac8I 283 GCAGGC GC[ACGT]^[ACGT]GC
615 GCTGGC
Cfr13I 625 GGTCC G^G[ACGT]CC
762 GGGCC
DpnII 361 GATC ^GATC
639 GATC
HphI 275 TCACC GGTGA(8/7)
293 TCACC
MaeIII 109 GTCAC ^GT[ACGT]AC
471 GTCAC
MboI 361 GATC ^GATC
639 GATC
MboII 39 TCTTC GAAGA(8/7)
182 TCTTC
NdeII 361 GATC ^GATC
639 GATC
Sau3AI 361 GATC ^GATC
639 GATC
Sau96I 625 GGTCC G^G[ACGT]CC
762 GGGCC
Tsp45I 109 GTCAC ^GT[GC]AC
471 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI BalI BbvI BsiYI
BslI BssKI BstF5I BstNI DdeI
EaeI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MluNI MnlI MscI
MseI MslI MvaI NlaIII NlaIV
PleI RsaI ScrFI SfaNI Tru9I
TseI Tsp509I TspRI