Restriction map for
pFN21AE9452
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BanI BanII BbeI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsoBI BspEI BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Alw44I 1324 GTGCAC G^TGCAC
ApaLI 1324 GTGCAC G^TGCAC
AseI 202 ATTAAT AT^TAAT
AsnI 202 ATTAAT AT^TAAT
BalI 1436 TGGCCA TGG^CCA
BamHI 1056 GGATCC G^GATCC
BbsI 904 GTCTTC GAAGAC(2/6)
BbvCI 1800 CCTCAGC CCTCAGC(-5/-2)
BcnI 495 CCGGG CC^[GC]GG
BfaI 517 CTAG C^TAG
BglI 1763 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1315 CTGGAG CTGGAG(16/14)
BpuAI 904 GTCTTC GAAGAC(2/6)
BsmBI 1095 CGTCTC CGTCTC(1/5)
BsmI 306 GCATTC GAATGC(1/-1)
Bsp1407I 211 TGTACA T^GTACA
BspDI 1481 ATCGAT AT^CGAT
BspHI 1385 TCATGA T^CATGA
BspLU11I 190 ACATGT A^CATGT
BsrFI 1267 GCCGGT [GA]^CCGG[CT]
BsrGI 211 TGTACA T^GTACA
BssSI 1219 CTCGTG CACGAG(-5/-1)
BstXI 1886 CCAGCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1267 GCCGGT [GA]^CCGG[CT]
ClaI 1481 ATCGAT AT^CGAT
DsaI 138 CCATGG C^C[GA][CT]GG
EcoNI 1497 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1423 CCCGC CCCGC(4/6)
MaeI 517 CTAG C^TAG
MfeI 603 CAATTG C^AATTG
MluNI 1436 TGGCCA TGG^CCA
MscI 1436 TGGCCA TGG^CCA
MunI 603 CAATTG C^AATTG
NciI 495 CCGGG CC^[GC]GG
NcoI 138 CCATGG C^CATGG
NspI 190 ACATGT [GA]CATG^[CT]
PflMI 1728 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1353 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 202 ATTAAT AT^TAAT
PvuII 1721 CAGCTG CAG^CTG
RcaI 1385 TCATGA T^CATGA
SgrAI 1266 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 211 TGTACA T^GTACA
StuI 24 AGGCCT AGG^CCT
Van91I 1728 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 516 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 913 GTCTAC GT^[AC][GT]AC
1789 GTCTAC
AcsI 412 AAATTT [GA]^AATT[CT]
1554 GAATTT
AfaI 212 GTAC GT^AC
893 GTAC
ApoI 412 AAATTT [GA]^AATT[CT]
1554 GAATTT
Asp700I 165 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1595 GAAAACTTTC
BbrPI 874 CACGTG CAC^GTG
973 CACGTG
BspMI 130 ACCTGC ACCTGC(4/8)
1809 GCAGGT
BsrDI 11 CATTGC GCAATG(2/0)
708 CATTGC
DraII 696 AGGTCCC [GA]G^G[ACGT]CC[CT]
1896 GGGTCCT
DraIII 990 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1689 CACATGGTG
EaeI 1292 TGGCCG [CT]^GGCC[GA]
1436 TGGCCA
EcoO109I 696 AGGTCCC [GA]G^G[ACGT]CC[CT]
1896 GGGTCCT
HapII 495 CCGG C^CGG
1268 CCGG
HgaI 858 GACGC GACGC(5/10)
1094 GCGTC
HincII 1360 GTCAAC GT[CT]^[GA]AC
1623 GTCAAC
HindII 1360 GTCAAC GT[CT]^[GA]AC
1623 GTCAAC
HpaII 495 CCGG C^CGG
1268 CCGG
MseI 203 TTAA T^TAA
242 TTAA
MspA1I 1424 CCGCTG C[AC]G^C[GT]G
1721 CAGCTG
MspI 495 CCGG C^CGG
1268 CCGG
PmaCI 874 CACGTG CAC^GTG
973 CACGTG
PmlI 874 CACGTG CAC^GTG
973 CACGTG
PpuMI 696 AGGTCCC [GA]G^G[AT]CC[CT]
1896 GGGTCCT
PstI 1177 CTGCAG CTGCA^G
1445 CTGCAG
RsaI 212 GTAC GT^AC
893 GTAC
SapI 921 GAAGAGC GCTCTTC(1/4)
1671 GCTCTTC
SmlI 587 CTCAAG C^T[CT][GA]AG
1368 CTCAAG
TfiI 505 GAATC G^A[AT]TC
1508 GATTC
Tru9I 203 TTAA T^TAA
242 TTAA
XmnI 165 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1595 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AflIII AluI AlwI AspHI
AvaII BbvI BlpI BmyI Bpu1102I
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII