Restriction map for
pFN21AE3025
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiHKAI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MflI MluI MroI MseI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
TspRI TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 112 GAATTC [GA]^AATT[CT]
AfaI 9 GTAC GT^AC
ApoI 112 GAATTC [GA]^AATT[CT]
AspI 279 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 74 CCCGGG C^[CT]CG[GA]G
AvaII 257 GGTCC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanII 144 GGGCTC G[GA]GC[CT]^C
BbsI 220 GAAGAC GAAGAC(2/6)
BbvCI 67 GCTGAGG CCTCAGC(-5/-2)
BglI 175 GCCCACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 144 GGGCTC G[AGT]GC[ACT]^C
BpuAI 220 GAAGAC GAAGAC(2/6)
BsiEI 105 CGGCCG CG[GA][CT]^CG
BsmAI 156 GAGAC GTCTC(1/5)
BsmBI 156 GAGACG CGTCTC(1/5)
BsoBI 74 CCCGGG C^[CT]CG[GA]G
Bsp1286I 144 GGGCTC G[AGT]GC[ACT]^C
BspMI 136 ACCTGC ACCTGC(4/8)
BssSI 178 CACGAG CACGAG(-5/-1)
BstAPI 377 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 82 GCGC GCG^C
DdeI 68 CTGAG C^T[ACGT]AG
DpnII 161 GATC ^GATC
DsaI 251 CCATGG C^C[GA][CT]GG
EagI 105 CGGCCG C^GGCCG
EclXI 105 CGGCCG C^GGCCG
Eco52I 105 CGGCCG C^GGCCG
EcoNI 296 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 112 GAATTC G^AATTC
EcoT14I 251 CCATGG C^C[AT][AT]GG
FauI 140 GCGGG CCCGC(4/6)
HaeII 81 AGCGCC [GA]GCGC^[CT]
HhaI 82 GCGC GCG^C
HinP1I 82 GCGC G^CGC
MaeIII 285 GTCAC ^GT[ACGT]AC
MboI 161 GATC ^GATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NcoI 251 CCATGG C^CATGG
NdeII 161 GATC ^GATC
NspI 11 ACATGC [GA]CATG^[CT]
PflFI 279 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 260 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 9 GTAC GT^AC
Sau3AI 161 GATC ^GATC
SmaI 74 CCCGGG CCC^GGG
StuI 293 AGGCCT AGG^CCT
StyI 251 CCATGG C^C[AT][AT]GG
Tsp45I 285 GTCAC ^GT[GC]AC
Tsp509I 113 AATT ^AATT
Tth111I 279 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 260 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 260 CCAGCGAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 74 CCCGGG C^CCGGG
XmaI 74 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 7 enzymes) #
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BsiYI 260 CCAGCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
296 CCTTTTACAGG
BslI 260 CCAGCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
296 CCTTTTACAGG
HphI 134 TCACC GGTGA(8/7)
286 TCACC
MspA1I 315 CAGCTG C[AC]G^C[GT]G
378 CAGCTG
NlaIII 12 CATG CATG^
252 CATG
PvuII 315 CAGCTG CAG^CTG
378 CAGCTG
SfaNI 205 GATGC GCATC(5/9)
211 GATGC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI AlwNI BbvI BcnI
BsaJI BssKI BstF5I BstNI Cac8I
Cfr13I EaeI EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MboII
MnlI MspI MvaI MwoI NciI
NlaIV Sau96I ScrFI TseI