Restriction map for
pFN21AE4352
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT14I FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI TfiI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 187 GTAC GT^AC
Alw44I 822 GTGCAC G^TGCAC
ApaI 668 GGGCCC GGGCC^C
ApaLI 822 GTGCAC G^TGCAC
AvaI 756 CCCGGG C^[CT]CG[GA]G
BclI 833 TGATCA T^GATCA
BpmI 136 CTGGAG CTGGAG(16/14)
BsaBI 305 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 340 GAGACC GGTCTC(1/5)
BsmFI 698 GTCCC GGGAC(10/14)
BsoBI 756 CCCGGG C^[CT]CG[GA]G
Bsp1407I 186 TGTACA T^GTACA
BspMI 503 ACCTGC ACCTGC(4/8)
BsrDI 867 CATTGC GCAATG(2/0)
BsrFI 154 ACCGGC [GA]^CCGG[CT]
BsrGI 186 TGTACA T^GTACA
BssHII 180 GCGCGC G^CGCGC
BstEII 384 GGTGACC G^GT[ACGT]ACC
BstPI 384 GGTGACC G^GT[ACGT]ACC
BstYI 986 AGATCC [GA]^GATC[CT]
Cfr10I 154 ACCGGC [GA]^CCGG[CT]
DraII 782 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 964 GACGAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 111 CCGCGG C^C[GA][CT]GG
EaeI 41 TGGCCG [CT]^GGCC[GA]
EcoO109I 782 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 384 GGTGACC G^GT[ACGT]ACC
EcoT22I 1087 ATGCAT ATGCA^T
FbaI 833 TGATCA T^GATCA
KspI 111 CCGCGG CCGC^GG
MamI 305 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 986 AGATCC [GA]^GATC[CT]
NsiI 1087 ATGCAT ATGCA^T
PflMI 1068 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 782 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 187 GTAC GT^AC
SacI 469 GAGCTC GAGCT^C
SacII 111 CCGCGG CCGC^GG
SmaI 756 CCCGGG CCC^GGG
SspBI 186 TGTACA T^GTACA
StuI 140 AGGCCT AGG^CCT
Van91I 1068 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 927 CCAGGAGTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 986 AGATCC [GA]^GATC[CT]
XmaCI 756 CCCGGG C^CCGGG
XmaI 756 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 456 CCTCAGG CC^T[ACGT]AGG
786 CCTGAGG
AspHI 469 GAGCTC G[AT]GC[AT]^C
822 GTGCAC
BanII 469 GAGCTC G[GA]GC[CT]^C
668 GGGCCC
BbeI 24 GGCGCC GGCGC^C
429 GGCGCC
BbvI 360 GCAGC GCAGC(8/12)
287 GCTGC
BsgI 288 CTGCAC GTGCAG(16/14)
358 GTGCAG
BsiHKAI 469 GAGCTC G[AT]GC[AT]^C
822 GTGCAC
BsmAI 340 GAGAC GTCTC(1/5)
973 GTCTC
BstXI 293 CCATTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
434 CCACATACTTGG
Bsu36I 456 CCTCAGG CC^T[ACGT]AGG
786 CCTGAGG
Eco81I 456 CCTCAGG CC^T[ACGT]AGG
786 CCTGAGG
HgaI 603 GACGC GACGC(5/10)
1024 GACGC
HinfI 652 GAGTC G^A[ACGT]TC
946 GACTC
HphI 384 GGTGA GGTGA(8/7)
570 GGTGA
KasI 24 GGCGCC G^GCGCC
429 GGCGCC
MaeII 67 ACGT A^CGT
377 ACGT
MaeIII 231 GTCAC ^GT[ACGT]AC
385 GTGAC
MslI 684 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
825 CACTGGGATG
NarI 24 GGCGCC GG^CGCC
429 GGCGCC
PleI 946 GACTC GAGTC(4/5)
652 GAGTC
PvuII 285 CAGCTG CAG^CTG
392 CAGCTG
SfoI 24 GGCGCC GGC^GCC
429 GGCGCC
TaqI 64 TCGA T^CGA
209 TCGA
TseI 287 GCTGC G^C[AT]GC
360 GCAGC
Tsp45I 231 GTCAC ^GT[GC]AC
385 GTGAC
Tsp509I 121 AATT ^AATT
523 AATT
TthHB8I 64 TCGA T^CGA
209 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaII BanI BbsI BcnI BmyI
BpuAI BsaHI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TspRI