Restriction map for
pFN21AE1809
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI BpmI Bpu1102I
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 783 GAATTT [GA]^AATT[CT]
AflIII 5 ACATGT A^C[GA][CT]GT
Aor51HI 383 AGCGCT AGC^GCT
ApoI 783 GAATTT [GA]^AATT[CT]
AspHI 637 GAGCAC G[AT]GC[AT]^C
BbsI 856 GAAGAC GAAGAC(2/6)
BbvCI 501 CCTCAGC CCTCAGC(-5/-2)
BglI 513 GCCTGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 374 AGATCT A^GATCT
BpuAI 856 GAAGAC GAAGAC(2/6)
BsaBI 721 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 262 GAGACC GGTCTC(1/5)
BsiHKAI 637 GAGCAC G[AT]GC[AT]^C
BsmBI 527 GAGACG CGTCTC(1/5)
BspLU11I 5 ACATGT A^CATGT
BspMI 737 ACCTGC ACCTGC(4/8)
BsrBI 288 CCGCTC CCGCTC(-3/-3)
BsrDI 344 GCAATG GCAATG(2/0)
BstAPI 732 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 435 GACCAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 89 CCGTGG C^C[GA][CT]GG
Eco47III 383 AGCGCT AGC^GCT
FauI 542 CCCGC CCCGC(4/6)
HinfI 859 GACTC G^A[ACGT]TC
HphI 94 GGTGA GGTGA(8/7)
MamI 721 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 856 GAAGA GAAGA(8/7)
MseI 944 TTAA T^TAA
NheI 318 GCTAGC G^CTAGC
NspI 5 ACATGT [GA]CATG^[CT]
PleI 859 GACTC GAGTC(4/5)
PstI 730 CTGCAG CTGCA^G
SfcI 730 CTGCAG C^T[GA][CT]AG
SmaI 134 CCCGGG CCC^GGG
SmlI 562 CTCAAG C^T[CT][GA]AG
StuI 923 AGGCCT AGG^CCT
TaqI 920 TCGA T^CGA
Tru9I 944 TTAA T^TAA
TthHB8I 920 TCGA T^CGA
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 312 GTAC GT^AC
735 GTAC
AlwI 177 GATCC GGATC(4/5)
426 GATCC
AlwNI 175 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
733 CAGTACCTG
BanI 61 GGTGCC G^G[CT][GA]CC
806 GGCACC
BcnI 134 CCCGG CC^[GC]GG
135 CCGGG
BfaI 205 CTAG C^TAG
319 CTAG
BmyI 304 GTGCCC G[AGT]GC[ACT]^C
637 GAGCAC
Bsp1286I 304 GTGCCC G[AGT]GC[ACT]^C
637 GAGCAC
BstF5I 24 CATCC GGATG(2/0)
195 CATCC
DraIII 476 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
863 CACTGGGTG
EcoT14I 152 CCAAGG C^C[AT][AT]GG
339 CCTTGG
FokI 24 CATCC GGATG(9/13)
195 CATCC
HaeII 15 GGCGCT [GA]GCGC^[CT]
383 AGCGCT
HapII 135 CCGG C^CGG
323 CCGG
HpaII 135 CCGG C^CGG
323 CCGG
MaeI 205 CTAG C^TAG
319 CTAG
MslI 225 CACTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
453 CATTTTTATG
MspA1I 550 CAGCTG C[AC]G^C[GT]G
571 CAGCTG
MspI 135 CCGG C^CGG
323 CCGG
NciI 134 CCCGG CC^[GC]GG
135 CCGGG
PflMI 380 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
651 CCACTGCCTGG
PpuMI 107 GGGTCCC [GA]G^G[AT]CC[CT]
818 AGGTCCC
PvuII 550 CAGCTG CAG^CTG
571 CAGCTG
RsaI 312 GTAC GT^AC
735 GTAC
StyI 152 CCAAGG C^C[AT][AT]GG
339 CCTTGG
Tsp509I 214 AATT ^AATT
784 AATT
Van91I 380 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
651 CCACTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AvaI AvaII BalI
BbvI BsaAI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI BsrI
BssKI BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI HaeIII HhaI
HinP1I ItaI MaeII MaeIII MboI
MflI MluNI MnlI MscI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI Tsp45I TspRI
XcmI XhoII