Restriction map for
pFN21AE2606
##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AvrII BbrPI BcgI BfrI
BlnI BlpI Bpu1102I BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoT22I FseI
HincII HindII HindIII HpaI KspI
MfeI MluI MunI NheI NotI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1496 GGTACC G^GTACC
AccIII 1007 TCCGGA T^CCGGA
AcyI 619 GGCGCC G[GA]^CG[CT]C
Asp700I 1734 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1496 GGTACC G^GTACC
AspHI 1074 GTGCTC G[AT]GC[AT]^C
BamHI 1841 GGATCC G^GATCC
BbeI 619 GGCGCC GGCGC^C
BbvCI 448 GCTGAGG CCTCAGC(-5/-2)
BfaI 643 CTAG C^TAG
BsaHI 619 GGCGCC G[GA]^CG[CT]C
BsaWI 1007 TCCGGA [AT]^CCGG[AT]
BseAI 1007 TCCGGA T^CCGGA
BseRI 1242 CTCCTC GAGGAG(10/8)
BsiHKAI 1074 GTGCTC G[AT]GC[AT]^C
BspEI 1007 TCCGGA T^CCGGA
BssHII 1523 GCGCGC G^CGCGC
BstEII 1095 GGTCACC G^GT[ACGT]ACC
BstPI 1095 GGTCACC G^GT[ACGT]ACC
BstXI 287 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 725 CCATGG C^C[GA][CT]GG
EagI 2042 CGGCCG C^GGCCG
EclXI 2042 CGGCCG C^GGCCG
Eco52I 2042 CGGCCG C^GGCCG
EcoO65I 1095 GGTCACC G^GT[ACGT]ACC
EcoRV 97 GATATC GAT^ATC
HgaI 1973 GCGTC GACGC(5/10)
Hin1I 619 GGCGCC G[GA]^CG[CT]C
KasI 619 GGCGCC G^GCGCC
KpnI 1496 GGTACC GGTAC^C
MaeI 643 CTAG C^TAG
MroI 1007 TCCGGA T^CCGGA
MseI 1673 TTAA T^TAA
NarI 619 GGCGCC GG^CGCC
NcoI 725 CCATGG C^CATGG
NdeI 102 CATATG CA^TATG
NruI 1025 TCGCGA TCG^CGA
NspI 1982 GCATGT [GA]CATG^[CT]
PaeR7I 1564 CTCGAG C^TCGAG
SapI 1467 GCTCTTC GCTCTTC(1/4)
SfoI 619 GGCGCC GGC^GCC
SmaI 386 CCCGGG CCC^GGG
SspI 825 AATATT AAT^ATT
TfiI 804 GATTC G^A[AT]TC
Tru9I 1673 TTAA T^TAA
XhoI 1564 CTCGAG C^TCGAG
XmaCI 386 CCCGGG C^CCGGG
XmaI 386 CCCGGG C^CCGGG
XmnI 1734 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AluI 382 AGCT AG^CT
1590 AGCT
AlwNI 1194 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1579 CAGCGGCTG
ApaI 1319 GGGCCC GGGCC^C
1430 GGGCCC
AvaI 386 CCCGGG C^[CT]CG[GA]G
1564 CTCGAG
AviII 665 TGCGCA TGC^GCA
1436 TGCGCA
BanII 1319 GGGCCC G[GA]GC[CT]^C
1430 GGGCCC
BbsI 1147 GTCTTC GAAGAC(2/6)
2064 GTCTTC
BclI 791 TGATCA T^GATCA
956 TGATCA
BglI 1048 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1126 GCCTACTGGGC
BpmI 1651 CTGGAG CTGGAG(16/14)
2023 CTGGAG
BpuAI 1147 GTCTTC GAAGAC(2/6)
2064 GTCTTC
BsaAI 139 TACGTG [CT]AC^GT[GA]
363 CACGTA
BsaBI 789 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1695 GATTGCCATC
BsaI 173 GAGACC GGTCTC(1/5)
1231 GAGACC
BsiEI 351 CGACCG CG[GA][CT]^CG
2042 CGGCCG
BsmBI 687 CGTCTC CGTCTC(1/5)
562 GAGACG
BsoBI 386 CCCGGG C^[CT]CG[GA]G
1564 CTCGAG
BsrBI 1465 CCGCTC CCGCTC(-3/-3)
10 GAGCGG
BsrDI 720 CATTGC GCAATG(2/0)
557 GCAATG
BsrFI 868 GCCGGC [GA]^CCGG[CT]
1455 GCCGGC
Cfr10I 868 GCCGGC [GA]^CCGG[CT]
1455 GCCGGC
EcoNI 609 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1724 CCTCGGCCAGG
EcoRI 307 GAATTC G^AATTC
1016 GAATTC
EcoT14I 500 CCTTGG C^C[AT][AT]GG
725 CCATGG
FbaI 791 TGATCA T^GATCA
956 TGATCA
FspI 665 TGCGCA TGC^GCA
1436 TGCGCA
MamI 789 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1695 GATTGCCATC
MspA1I 1579 CAGCGG C[AC]G^C[GT]G
2108 CAGCGG
NaeI 868 GCCGGC GCC^GGC
1455 GCCGGC
NgoMIV 868 GCCGGC G^CCGGC
1455 GCCGGC
PpuMI 69 AGGACCC [GA]G^G[AT]CC[CT]
2018 AGGACCT
PstI 1333 CTGCAG CTGCA^G
1592 CTGCAG
ScaI 398 AGTACT AGT^ACT
845 AGTACT
SexAI 608 ACCTGGT A^CC[AT]GGT
940 ACCTGGT
SmlI 1564 CTCGAG C^T[CT][GA]AG
2074 CTCAAG
StuI 711 AGGCCT AGG^CCT
2050 AGGCCT
StyI 500 CCTTGG C^C[AT][AT]GG
725 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AlwI
ApoI AvaII BalI BanI BbvI
BciVI BcnI BglII BmyI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII