Restriction map for
pFN21AB7153
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BanI BbeI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BseRI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XhoII
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 837 GAATTC [GA]^AATT[CT]
AflIII 863 ACACGT A^C[GA][CT]GT
AlwI 795 GATCC GGATC(4/5)
ApoI 837 GAATTC [GA]^AATT[CT]
Asp700I 148 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 160 GTGCTC G[AT]GC[AT]^C
AviII 629 TGCGCA TGC^GCA
BanII 327 GGGCTC G[GA]GC[CT]^C
BbsI 358 GTCTTC GAAGAC(2/6)
BbvCI 291 GCTGAGG CCTCAGC(-5/-2)
BglI 610 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 358 GTCTTC GAAGAC(2/6)
BsgI 307 GTGCAG GTGCAG(16/14)
BsiHKAI 160 GTGCTC G[AT]GC[AT]^C
BspMI 881 ACCTGC ACCTGC(4/8)
BsrBI 648 CCGCTC CCGCTC(-3/-3)
BsrI 240 ACTGG ACTGG(1/-1)
BssSI 462 CTCGTG CACGAG(-5/-1)
DdeI 292 CTGAG C^T[ACGT]AG
EcoRI 837 GAATTC G^AATTC
FspI 629 TGCGCA TGC^GCA
HaeII 386 AGCGCC [GA]GCGC^[CT]
MspA1I 904 CCGCTG C[AC]G^C[GT]G
NheI 684 GCTAGC G^CTAGC
PaeR7I 526 CTCGAG C^TCGAG
PstI 907 CTGCAG CTGCA^G
SfcI 907 CTGCAG C^T[GA][CT]AG
SfiI 609 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 530 AGGCCT AGG^CCT
TaqI 527 TCGA T^CGA
TthHB8I 527 TCGA T^CGA
XhoI 526 CTCGAG C^TCGAG
XmnI 148 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 300 GACGCC G[GA]^CG[CT]C
718 GGCGTC
BbrPI 391 CACGTG CAC^GTG
864 CACGTG
BfaI 685 CTAG C^TAG
715 CTAG
BmyI 160 GTGCTC G[AGT]GC[ACT]^C
327 GGGCTC
BsaHI 300 GACGCC G[GA]^CG[CT]C
718 GGCGTC
BsiEI 901 CGGCCG CG[GA][CT]^CG
919 CGGCCG
Bsp1286I 160 GTGCTC G[AGT]GC[ACT]^C
327 GGGCTC
BstAPI 564 GCATTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
632 GCATCTACTGC
BstEII 582 GGTGACC G^GT[ACGT]ACC
702 GGTCACC
BstPI 582 GGTGACC G^GT[ACGT]ACC
702 GGTCACC
DpnII 235 GATC ^GATC
795 GATC
EagI 901 CGGCCG C^GGCCG
919 CGGCCG
EclXI 901 CGGCCG C^GGCCG
919 CGGCCG
Eco52I 901 CGGCCG C^GGCCG
919 CGGCCG
EcoO65I 582 GGTGACC G^GT[ACGT]ACC
702 GGTCACC
Hin1I 300 GACGCC G[GA]^CG[CT]C
718 GGCGTC
MaeI 685 CTAG C^TAG
715 CTAG
MboI 235 GATC ^GATC
795 GATC
MboII 359 TCTTC GAAGA(8/7)
590 TCTTC
NdeII 235 GATC ^GATC
795 GATC
PmaCI 391 CACGTG CAC^GTG
864 CACGTG
PmlI 391 CACGTG CAC^GTG
864 CACGTG
PpuMI 878 GGGACCT [GA]G^G[AT]CC[CT]
955 GGGACCC
Sau3AI 235 GATC ^GATC
795 GATC
SfaNI 632 GCATC GCATC(5/9)
752 GCATC
SmaI 320 CCCGGG CCC^GGG
960 CCCGGG
SphI 440 GCATGC GCATG^C
1010 GCATGC
Tsp509I 55 AATT ^AATT
838 AATT
XcmI 62 CCAAGGAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
596 CCATCATCCTGTTGG
XmaCI 320 CCCGGG C^CCGGG
960 CCCGGG
XmaI 320 CCCGGG C^CCGGG
960 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BalI BbvI BcnI BpmI BsaAI
BsaJI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI BsrDI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NlaIII NlaIV NspI RsaI
Sau96I ScrFI SmlI StyI TseI
Tsp45I TspRI