Restriction map for
pFN21AE3987
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AvrII BbeI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglI BlnI BpuAI BsaAI BsaHI
BsaI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
Hin1I HindIII HpaI KasI KspI
MaeI MaeII MfeI MluI MroI
MseI MslI MunI NarI NdeI
NheI NotI NruI PacI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 553 GGTACC G^GTACC
AccI 139 GTCGAC GT^[AC][GT]AC
AflIII 135 ACATGT A^C[GA][CT]GT
AgeI 119 ACCGGT A^CCGGT
AhdI 164 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 553 GGTACC G^GTACC
AspEI 164 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 37 CTCGAG C^[CT]CG[GA]G
AviII 689 TGCGCA TGC^GCA
BalI 662 TGGCCA TGG^CCA
BamHI 71 GGATCC G^GATCC
BcgI 180 GCACCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 600 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 600 GCTCAGC GC^T[ACGT]AGC
BsaBI 72 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 825 CTCCTC GAGGAG(10/8)
BsiEI 117 CGACCG CG[GA][CT]^CG
BsmAI 571 GAGAC GTCTC(1/5)
BsoBI 37 CTCGAG C^[CT]CG[GA]G
BspLU11I 135 ACATGT A^CATGT
BspMI 503 ACCTGC ACCTGC(4/8)
BstBI 188 TTCGAA TT^CGAA
CelII 600 GCTCAGC GC^T[ACGT]AGC
DsaI 410 CCATGG C^C[GA][CT]GG
Eam1105I 164 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 102 GAAGAG CTCTTC(1/4)
EcoNI 906 CCTGTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 410 CCATGG C^C[AT][AT]GG
EcoT22I 462 ATGCAT ATGCA^T
FauI 484 CCCGC CCCGC(4/6)
FspI 689 TGCGCA TGC^GCA
HaeII 314 AGCGCC [GA]GCGC^[CT]
HincII 139 GTCGAC GT[CT]^[GA]AC
HindII 139 GTCGAC GT[CT]^[GA]AC
KpnI 553 GGTACC GGTAC^C
Ksp632I 102 GAAGAG CTCTTC(1/4)
MamI 72 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 662 TGGCCA TGG^CCA
MscI 662 TGGCCA TGG^CCA
NaeI 753 GCCGGC GCC^GGC
NcoI 410 CCATGG C^CATGG
NgoMIV 753 GCCGGC G^CCGGC
NsiI 462 ATGCAT ATGCA^T
NspV 188 TTCGAA TT^CGAA
PaeR7I 37 CTCGAG C^TCGAG
PinAI 119 ACCGGT A^CCGGT
PshAI 6 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 396 CAGCTG CAG^CTG
SacI 598 GAGCTC GAGCT^C
SalI 139 GTCGAC G^TCGAC
SfuI 188 TTCGAA TT^CGAA
StyI 410 CCATGG C^C[AT][AT]GG
XcmI 953 CCAAAGAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 37 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 478 CGCG CG^CG
518 CGCG
AspHI 499 GAGCAC G[AT]GC[AT]^C
598 GAGCTC
AspI 25 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
475 GACCGCGTC
BanII 284 GAGCCC G[GA]GC[CT]^C
598 GAGCTC
BglII 41 AGATCT A^GATCT
404 AGATCT
BsaWI 68 ACCGGA [AT]^CCGG[AT]
119 ACCGGT
BsgI 391 CTGCAC GTGCAG(16/14)
633 CTGCAC
BsiHKAI 499 GAGCAC G[AT]GC[AT]^C
598 GAGCTC
BsmI 460 GAATGC GAATGC(1/-1)
749 GAATGC
BsrFI 119 ACCGGT [GA]^CCGG[CT]
753 GCCGGC
BsrI 968 ACTGG ACTGG(1/-1)
261 CCAGT
BstUI 478 CGCG CG^CG
518 CGCG
Cfr10I 119 ACCGGT [GA]^CCGG[CT]
753 GCCGGC
EaeI 332 TGGCCG [CT]^GGCC[GA]
662 TGGCCA
MspA1I 96 CCGCTG C[AC]G^C[GT]G
396 CAGCTG
MvnI 478 CGCG CG^CG
518 CGCG
NspI 135 ACATGT [GA]CATG^[CT]
327 GCATGT
PflFI 25 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
475 GACCGCGTC
PflMI 410 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
924 CCACTGTATGG
TspRI 813 CACTG CA[GC]TG[ACGT][ACGT]^
925 CACTG
Tth111I 25 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
475 GACCGCGTC
Van91I 410 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
924 CCACTGTATGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmlI Sse8387I TaqI TseI
Tsp45I TthHB8I XhoII