Restriction map for
pFN21AE1150
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1394 GTAGAC GT^[AC][GT]AC
AflII 397 CTTAAG C^TTAAG
AflIII 978 ACATGT A^C[GA][CT]GT
Alw44I 472 GTGCAC G^TGCAC
AlwI 863 GATCC GGATC(4/5)
ApaLI 472 GTGCAC G^TGCAC
AspHI 472 GTGCAC G[AT]GC[AT]^C
AvaI 1047 CTCGGG C^[CT]CG[GA]G
BalI 1026 TGGCCA TGG^CCA
BbvI 4 GCAGC GCAGC(8/12)
BciVI 314 GGATAC GTATCC(6/5)
BclI 891 TGATCA T^GATCA
BfrI 397 CTTAAG C^TTAAG
BmyI 472 GTGCAC G[AGT]GC[ACT]^C
BsaWI 1330 ACCGGA [AT]^CCGG[AT]
BseRI 157 GAGGAG GAGGAG(10/8)
BsiHKAI 472 GTGCAC G[AT]GC[AT]^C
BsmFI 18 GTCCC GGGAC(10/14)
BsmI 1432 GAATGC GAATGC(1/-1)
BsoBI 1047 CTCGGG C^[CT]CG[GA]G
Bsp1286I 472 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 555 TGTACA T^GTACA
BspLU11I 978 ACATGT A^CATGT
BspMI 1316 ACCTGC ACCTGC(4/8)
BsrGI 555 TGTACA T^GTACA
BstXI 154 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 862 AGATCC [GA]^GATC[CT]
Cac8I 628 GCAAGC GC[ACGT]^[ACGT]GC
Cfr13I 1050 GGGCC G^G[ACGT]CC
DsaI 21 CCGTGG C^C[GA][CT]GG
EaeI 1026 TGGCCA [CT]^GGCC[GA]
FbaI 891 TGATCA T^GATCA
Fnu4HI 4 GCAGC GC^[ACGT]GC
HapII 1331 CCGG C^CGG
HpaII 1331 CCGG C^CGG
ItaI 4 GCAGC GC^[ACGT]GC
MaeII 124 ACGT A^CGT
MflI 862 AGATCC [GA]^GATC[CT]
MluNI 1026 TGGCCA TGG^CCA
MscI 1026 TGGCCA TGG^CCA
MspI 1331 CCGG C^CGG
MwoI 697 GCTGTCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 643 CATATG CA^TATG
NlaIV 39 GGAACC GG[ACGT]^[ACGT]CC
NspI 978 ACATGT [GA]CATG^[CT]
Sau96I 1050 GGGCC G^G[ACGT]CC
ScaI 1043 AGTACT AGT^ACT
SfaNI 207 GATGC GCATC(5/9)
SmlI 397 CTTAAG C^T[CT][GA]AG
SspBI 555 TGTACA T^GTACA
SspI 303 AATATT AAT^ATT
TseI 4 GCAGC G^C[AT]GC
XhoII 862 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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AluI 696 AGCT AG^CT
705 AGCT
BbsI 1197 GAAGAC GAAGAC(2/6)
376 GTCTTC
BfaI 257 CTAG C^TAG
956 CTAG
BpmI 542 CTCCAG CTGGAG(16/14)
211 CTGGAG
BpuAI 1197 GAAGAC GAAGAC(2/6)
376 GTCTTC
BsaJI 21 CCGTGG C^C[ACGT][ACGT]GG
35 CCAGGG
EcoRI 1154 GAATTC G^AATTC
1406 GAATTC
HphI 149 TCACC GGTGA(8/7)
1314 TCACC
MaeI 257 CTAG C^TAG
956 CTAG
MslI 655 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
923 CACACAAGTG
SfcI 195 CTACAG C^T[GA][CT]AG
333 CTACAG
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AfaI ApoI Asp700I BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII FokI HaeIII HinfI MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII RsaI Sau3AI ScrFI
TfiI Tru9I Tsp45I Tsp509I TspRI
XmnI