Restriction map for
pFN21AE1715
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanII BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MboII MfeI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AcyI 256 GGCGCC G[GA]^CG[CT]C
AlwNI 646 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 204 TGGCCA TGG^CCA
BanI 256 GGCGCC G^G[CT][GA]CC
BbeI 256 GGCGCC GGCGC^C
BbvCI 458 CCTCAGC CCTCAGC(-5/-2)
BpmI 112 CTGGAG CTGGAG(16/14)
BsaHI 256 GGCGCC G[GA]^CG[CT]C
BsaI 454 GAGACC GGTCTC(1/5)
BsgI 248 GTGCAG GTGCAG(16/14)
BsiYI 387 CCACCTGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 387 CCACCTGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 454 GAGAC GTCTC(1/5)
BsmI 490 GCATTC GAATGC(1/-1)
BstXI 128 CCATCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 4 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 204 TGGCCA [CT]^GGCC[GA]
EcoO109I 4 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 464 CCTTGG C^C[AT][AT]GG
Hin1I 256 GGCGCC G[GA]^CG[CT]C
KasI 256 GGCGCC G^GCGCC
MluNI 204 TGGCCA TGG^CCA
MscI 204 TGGCCA TGG^CCA
MspA1I 200 CCGCTG C[AC]G^C[GT]G
NarI 256 GGCGCC GG^CGCC
NlaIII 357 CATG CATG^
PpuMI 4 GGGACCC [GA]G^G[AT]CC[CT]
PstI 634 CTGCAG CTGCA^G
SfcI 634 CTGCAG C^T[GA][CT]AG
SfoI 256 GGCGCC GGC^GCC
StyI 464 CCTTGG C^C[AT][AT]GG
TaqI 452 TCGA T^CGA
TfiI 469 GATTC G^A[AT]TC
TthHB8I 452 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AluI 323 AGCT AG^CT
632 AGCT
Aor51HI 212 AGCGCT AGC^GCT
308 AGCGCT
AspHI 43 GTGCTC G[AT]GC[AT]^C
123 GAGCAC
AvaI 8 CCCGGG C^[CT]CG[GA]G
570 CCCGGG
AvaII 5 GGACC G^G[AT]CC
330 GGACC
BglII 188 AGATCT A^GATCT
512 AGATCT
BsiHKAI 43 GTGCTC G[AT]GC[AT]^C
123 GAGCAC
BsoBI 8 CCCGGG C^[CT]CG[GA]G
570 CCCGGG
BstF5I 156 CATCC GGATG(2/0)
501 CATCC
BstYI 188 AGATCT [GA]^GATC[CT]
512 AGATCT
DdeI 57 CTCAG C^T[ACGT]AG
459 CTCAG
DsaI 243 CCGTGG C^C[GA][CT]GG
608 CCACGG
Eco47III 212 AGCGCT AGC^GCT
308 AGCGCT
FokI 156 CATCC GGATG(9/13)
501 CATCC
HaeIII 205 GGCC GG^CC
414 GGCC
HapII 9 CCGG C^CGG
571 CCGG
HpaII 9 CCGG C^CGG
571 CCGG
HphI 92 TCACC GGTGA(8/7)
227 TCACC
MaeIII 377 GTGAC ^GT[ACGT]AC
528 GTCAC
MflI 188 AGATCT [GA]^GATC[CT]
512 AGATCT
MspI 9 CCGG C^CGG
571 CCGG
PleI 55 GACTC GAGTC(4/5)
565 GAGTC
SfaNI 155 GCATC GCATC(5/9)
521 GCATC
SmaI 8 CCCGGG CCC^GGG
570 CCCGGG
Tsp45I 377 GTGAC ^GT[GC]AC
528 GTCAC
XhoII 188 AGATCT [GA]^GATC[CT]
512 AGATCT
XmaCI 8 CCCGGG C^CCGGG
570 CCCGGG
XmaI 8 CCCGGG C^CCGGG
570 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AfaI BbvI BcnI
BmyI BsaJI BsmFI Bsp1286I BspMI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII FauI Fnu4HI
HaeII HhaI HinP1I HinfI ItaI
MaeII MboI MnlI MslI MvaI
MvnI MwoI NciI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI
TspRI