Restriction map for
pFN21AE4386
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BanII BbrPI
BbvCI BcgI BciVI BclI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BseRI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI Eam1105I EcoNI EcoO65I EcoRV
EcoT22I FbaI FspI HpaI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 407 GAATTC [GA]^AATT[CT]
AflII 1120 CTTAAG C^TTAAG
Alw44I 871 GTGCAC G^TGCAC
Aor51HI 64 AGCGCT AGC^GCT
ApaLI 871 GTGCAC G^TGCAC
ApoI 407 GAATTC [GA]^AATT[CT]
AvaI 496 CCCGAG C^[CT]CG[GA]G
BalI 467 TGGCCA TGG^CCA
BbsI 1140 GAAGAC GAAGAC(2/6)
BfaI 454 CTAG C^TAG
BfrI 1120 CTTAAG C^TTAAG
BlpI 195 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 195 GCTGAGC GC^T[ACGT]AGC
BpuAI 1140 GAAGAC GAAGAC(2/6)
BsaI 623 GGTCTC GGTCTC(1/5)
BsiEI 17 CGGCCG CG[GA][CT]^CG
BsmAI 624 GTCTC GTCTC(1/5)
BsoBI 496 CCCGAG C^[CT]CG[GA]G
BssHII 200 GCGCGC G^CGCGC
CelII 195 GCTGAGC GC^T[ACGT]AGC
DraII 299 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 361 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 292 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 17 CGGCCG C^GGCCG
EarI 355 CTCTTC CTCTTC(1/4)
EclXI 17 CGGCCG C^GGCCG
Eco47III 64 AGCGCT AGC^GCT
Eco52I 17 CGGCCG C^GGCCG
EcoO109I 299 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 407 GAATTC G^AATTC
EcoT14I 1196 CCATGG C^C[AT][AT]GG
FseI 924 GGCCGGCC GGCCGG^CC
HgaI 106 GCGTC GACGC(5/10)
HincII 514 GTTGAC GT[CT]^[GA]AC
HindII 514 GTTGAC GT[CT]^[GA]AC
HindIII 1117 AAGCTT A^AGCTT
HphI 639 GGTGA GGTGA(8/7)
Ksp632I 355 CTCTTC CTCTTC(1/4)
MaeI 454 CTAG C^TAG
MluNI 467 TGGCCA TGG^CCA
MscI 467 TGGCCA TGG^CCA
MseI 1121 TTAA T^TAA
NaeI 925 GCCGGC GCC^GGC
NcoI 1196 CCATGG C^CATGG
NgoMIV 925 GCCGGC G^CCGGC
PleI 140 GACTC GAGTC(4/5)
PpuMI 299 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 1085 AGTACT AGT^ACT
StyI 1196 CCATGG C^C[AT][AT]GG
TfiI 1130 GAATC G^A[AT]TC
Tru9I 1121 TTAA T^TAA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 970 GTAC GT^AC
1086 GTAC
AocI 295 CCTGAGG CC^T[ACGT]AGG
616 CCTCAGG
AspHI 8 GTGCTC G[AT]GC[AT]^C
871 GTGCAC
BamHI 344 GGATCC G^GATCC
747 GGATCC
BmyI 8 GTGCTC G[AGT]GC[ACT]^C
871 GTGCAC
BsiHKAI 8 GTGCTC G[AT]GC[AT]^C
871 GTGCAC
Bsp1286I 8 GTGCTC G[AGT]GC[ACT]^C
871 GTGCAC
BspMI 401 ACCTGC ACCTGC(4/8)
1057 ACCTGC
BstAPI 396 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
543 GCACCGGCTGC
BstF5I 1098 CATCC GGATG(2/0)
840 GGATG
BstYI 344 GGATCC [GA]^GATC[CT]
747 GGATCC
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
616 CCTCAGG
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
616 CCTCAGG
FokI 1098 CATCC GGATG(9/13)
840 GGATG
HinfI 140 GACTC G^A[ACGT]TC
1130 GAATC
MflI 344 GGATCC [GA]^GATC[CT]
747 GGATCC
RsaI 970 GTAC GT^AC
1086 GTAC
SmlI 325 CTCAAG C^T[CT][GA]AG
1120 CTTAAG
Tsp509I 408 AATT ^AATT
678 AATT
XhoII 344 GGATCC [GA]^GATC[CT]
747 GGATCC
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaII BanI BbeI BbvI
BcnI BglI BpmI BsaHI BsaJI
BsgI BsiYI BslI BsmFI BsrBI
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI KasI
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI TseI TspRI
Van91I