################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI AhdI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanII BbrPI BbvCI BcgI BciVI BclI BglII BlnI BsaAI BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI Eam1105I EcoNI EcoO65I EcoRV EcoT22I FbaI FspI HpaI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcsI 407 GAATTC [GA]^AATT[CT] AflII 1120 CTTAAG C^TTAAG Alw44I 871 GTGCAC G^TGCAC Aor51HI 64 AGCGCT AGC^GCT ApaLI 871 GTGCAC G^TGCAC ApoI 407 GAATTC [GA]^AATT[CT] AvaI 496 CCCGAG C^[CT]CG[GA]G BalI 467 TGGCCA TGG^CCA BbsI 1140 GAAGAC GAAGAC(2/6) BfaI 454 CTAG C^TAG BfrI 1120 CTTAAG C^TTAAG BlpI 195 GCTGAGC GC^T[ACGT]AGC Bpu1102I 195 GCTGAGC GC^T[ACGT]AGC BpuAI 1140 GAAGAC GAAGAC(2/6) BsaI 623 GGTCTC GGTCTC(1/5) BsiEI 17 CGGCCG CG[GA][CT]^CG BsmAI 624 GTCTC GTCTC(1/5) BsoBI 496 CCCGAG C^[CT]CG[GA]G BssHII 200 GCGCGC G^CGCGC CelII 195 GCTGAGC GC^T[ACGT]AGC DraII 299 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 361 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 292 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 17 CGGCCG C^GGCCG EarI 355 CTCTTC CTCTTC(1/4) EclXI 17 CGGCCG C^GGCCG Eco47III 64 AGCGCT AGC^GCT Eco52I 17 CGGCCG C^GGCCG EcoO109I 299 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 407 GAATTC G^AATTC EcoT14I 1196 CCATGG C^C[AT][AT]GG FseI 924 GGCCGGCC GGCCGG^CC HgaI 106 GCGTC GACGC(5/10) HincII 514 GTTGAC GT[CT]^[GA]AC HindII 514 GTTGAC GT[CT]^[GA]AC HindIII 1117 AAGCTT A^AGCTT HphI 639 GGTGA GGTGA(8/7) Ksp632I 355 CTCTTC CTCTTC(1/4) MaeI 454 CTAG C^TAG MluNI 467 TGGCCA TGG^CCA MscI 467 TGGCCA TGG^CCA MseI 1121 TTAA T^TAA NaeI 925 GCCGGC GCC^GGC NcoI 1196 CCATGG C^CATGG NgoMIV 925 GCCGGC G^CCGGC PleI 140 GACTC GAGTC(4/5) PpuMI 299 AGGTCCT [GA]G^G[AT]CC[CT] ScaI 1085 AGTACT AGT^ACT StyI 1196 CCATGG C^C[AT][AT]GG TfiI 1130 GAATC G^A[AT]TC Tru9I 1121 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 970 GTAC GT^AC 1086 GTAC AocI 295 CCTGAGG CC^T[ACGT]AGG 616 CCTCAGG AspHI 8 GTGCTC G[AT]GC[AT]^C 871 GTGCAC BamHI 344 GGATCC G^GATCC 747 GGATCC BmyI 8 GTGCTC G[AGT]GC[ACT]^C 871 GTGCAC BsiHKAI 8 GTGCTC G[AT]GC[AT]^C 871 GTGCAC Bsp1286I 8 GTGCTC G[AGT]GC[ACT]^C 871 GTGCAC BspMI 401 ACCTGC ACCTGC(4/8) 1057 ACCTGC BstAPI 396 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 543 GCACCGGCTGC BstF5I 1098 CATCC GGATG(2/0) 840 GGATG BstYI 344 GGATCC [GA]^GATC[CT] 747 GGATCC Bsu36I 295 CCTGAGG CC^T[ACGT]AGG 616 CCTCAGG Eco81I 295 CCTGAGG CC^T[ACGT]AGG 616 CCTCAGG FokI 1098 CATCC GGATG(9/13) 840 GGATG HinfI 140 GACTC G^A[ACGT]TC 1130 GAATC MflI 344 GGATCC [GA]^GATC[CT] 747 GGATCC RsaI 970 GTAC GT^AC 1086 GTAC SmlI 325 CTCAAG C^T[CT][GA]AG 1120 CTTAAG Tsp509I 408 AATT ^AATT 678 AATT XhoII 344 GGATCC [GA]^GATC[CT] 747 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI AvaII BanI BbeI BbvI BcnI BglI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmFI BsrBI BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI TseI TspRI Van91I