Restriction map for
pFN21AE5135
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DraIII DrdI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
NarI NciI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 262 ACATGT A^C[GA][CT]GT
AhdI 258 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 6 AGCT AG^CT
AspEI 258 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 63 GTGCTC G[AT]GC[AT]^C
AvaI 191 CCCGAG C^[CT]CG[GA]G
BamHI 92 GGATCC G^GATCC
BfaI 50 CTAG C^TAG
BglI 188 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 63 GTGCTC G[AGT]GC[ACT]^C
BsaI 256 GAGACC GGTCTC(1/5)
BsaWI 295 ACCGGA [AT]^CCGG[AT]
BsiHKAI 63 GTGCTC G[AT]GC[AT]^C
BsmFI 173 GGGAC GGGAC(10/14)
BsoBI 191 CCCGAG C^[CT]CG[GA]G
Bsp1286I 63 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 262 ACATGT A^CATGT
BssSI 237 CTCGTG CACGAG(-5/-1)
BstF5I 243 GGATG GGATG(2/0)
BstYI 92 GGATCC [GA]^GATC[CT]
DpnII 93 GATC ^GATC
DraII 173 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 68 CCATGG C^C[GA][CT]GG
Eam1105I 258 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 173 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 68 CCATGG C^C[AT][AT]GG
FauI 280 GCGGG CCCGC(4/6)
FokI 243 GGATG GGATG(9/13)
HaeIII 142 GGCC GG^CC
HgaI 158 GCGTC GACGC(5/10)
MaeI 50 CTAG C^TAG
MboI 93 GATC ^GATC
MboII 73 GAAGA GAAGA(8/7)
MflI 92 GGATCC [GA]^GATC[CT]
NaeI 106 GCCGGC GCC^GGC
NcoI 68 CCATGG C^CATGG
NdeII 93 GATC ^GATC
NgoMIV 106 GCCGGC G^CCGGC
NspI 262 ACATGT [GA]CATG^[CT]
PpuMI 173 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 93 GATC ^GATC
SspI 117 AATATT AAT^ATT
StyI 68 CCATGG C^C[AT][AT]GG
XcmI 167 CCAGGGGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 92 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AciI 227 CCGC CCGC(-3/-1)
280 GCGG
AlwI 93 GATCC GGATC(4/5)
92 GGATC
AvaII 174 GGACC G^G[AT]CC
293 GGACC
BpmI 165 CTCCAG CTGGAG(16/14)
145 CTGGAG
BsgI 213 CTGCAC GTGCAG(16/14)
316 GTGCAG
BsmAI 256 GAGAC GTCTC(1/5)
14 GTCTC
BsrFI 106 GCCGGC [GA]^CCGG[CT]
208 ACCGGC
BsrI 290 ACTGG ACTGG(1/-1)
273 CCAGT
BssHII 249 GCGCGC G^CGCGC
301 GCGCGC
Cfr10I 106 GCCGGC [GA]^CCGG[CT]
208 ACCGGC
Cfr13I 174 GGACC G^G[ACGT]CC
293 GGACC
HphI 161 TCACC GGTGA(8/7)
206 TCACC
Sau96I 174 GGACC G^G[ACGT]CC
293 GGACC
TspRI 268 CACTG CA[GC]TG[ACGT][ACGT]^
274 CAGTG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AccII BbvI BsaJI BseRI BsiYI
BslI BssKI BstNI BstUI Cac8I
CfoI EcoRII Fnu4HI HapII HhaI
HinP1I HpaII ItaI MaeIII MnlI
MspI MvaI MvnI MwoI NlaIII
NlaIV ScrFI TseI Tsp45I