Restriction map for
pFN21AE1678
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BpuAI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MflI MluI
MroI MseI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 81 CGCG CG^CG
AcyI 474 GACGCC G[GA]^CG[CT]C
Aor51HI 116 AGCGCT AGC^GCT
ApaI 231 GGGCCC GGGCC^C
BalI 335 TGGCCA TGG^CCA
BanI 365 GGCACC G^G[CT][GA]CC
BanII 231 GGGCCC G[GA]GC[CT]^C
BbvCI 199 CCTCAGC CCTCAGC(-5/-2)
BciVI 290 GTATCC GTATCC(6/5)
BmyI 231 GGGCCC G[AGT]GC[ACT]^C
BpmI 360 CTCCAG CTGGAG(16/14)
BsaAI 287 TACGTA [CT]AC^GT[GA]
BsaHI 474 GACGCC G[GA]^CG[CT]C
BsaI 547 GAGACC GGTCTC(1/5)
BsmBI 430 CGTCTC CGTCTC(1/5)
Bsp1286I 231 GGGCCC G[AGT]GC[ACT]^C
BsrDI 456 CATTGC GCAATG(2/0)
BssSI 100 CTCGTG CACGAG(-5/-1)
BstUI 81 CGCG CG^CG
DpnII 93 GATC ^GATC
Eco47III 116 AGCGCT AGC^GCT
HapII 4 CCGG C^CGG
Hin1I 474 GACGCC G[GA]^CG[CT]C
HpaII 4 CCGG C^CGG
MboI 93 GATC ^GATC
MluNI 335 TGGCCA TGG^CCA
MscI 335 TGGCCA TGG^CCA
MspI 4 CCGG C^CGG
MvnI 81 CGCG CG^CG
NcoI 258 CCATGG C^CATGG
NdeII 93 GATC ^GATC
PaeR7I 148 CTCGAG C^TCGAG
PleI 151 GAGTC GAGTC(4/5)
PstI 178 CTGCAG CTGCA^G
Sau3AI 93 GATC ^GATC
SfcI 178 CTGCAG C^T[GA][CT]AG
SmlI 148 CTCGAG C^T[CT][GA]AG
SnaBI 287 TACGTA TAC^GTA
TaqI 149 TCGA T^CGA
TthHB8I 149 TCGA T^CGA
XcmI 377 CCACCCTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 148 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
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AfaI 316 GTAC GT^AC
452 GTAC
AlwNI 136 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
466 CAGCGCCTG
AvaI 148 CTCGAG C^[CT]CG[GA]G
485 CCCGAG
BlpI 130 GCTCAGC GC^T[ACGT]AGC
172 GCTGAGC
Bpu1102I 130 GCTCAGC GC^T[ACGT]AGC
172 GCTGAGC
BseRI 345 CTCCTC GAGGAG(10/8)
7 GAGGAG
BsgI 501 CTGCAC GTGCAG(16/14)
562 GTGCAG
BsmAI 547 GAGAC GTCTC(1/5)
431 GTCTC
BsoBI 148 CTCGAG C^[CT]CG[GA]G
485 CCCGAG
BsrBI 145 CCGCTC CCGCTC(-3/-3)
246 CCGCTC
BstAPI 135 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
564 GCAGTGGGTGC
BstF5I 111 CATCC GGATG(2/0)
321 CATCC
BstXI 294 CCATCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
374 CCACCACCCTGG
CelII 130 GCTCAGC GC^T[ACGT]AGC
172 GCTGAGC
DsaI 258 CCATGG C^C[GA][CT]GG
386 CCGTGG
EaeI 335 TGGCCA [CT]^GGCC[GA]
383 TGGCCG
EcoT14I 258 CCATGG C^C[AT][AT]GG
444 CCAAGG
FokI 111 CATCC GGATG(9/13)
321 CATCC
HaeII 116 AGCGCT [GA]GCGC^[CT]
467 AGCGCC
HphI 90 GGTGA GGTGA(8/7)
327 GGTGA
MaeII 167 ACGT A^CGT
288 ACGT
MaeIII 153 GTCAC ^GT[ACGT]AC
328 GTGAC
MspA1I 139 CAGCTG C[AC]G^C[GT]G
181 CAGCTG
NlaIII 259 CATG CATG^
312 CATG
PpuMI 195 AGGACCT [GA]G^G[AT]CC[CT]
206 AGGACCC
PvuII 139 CAGCTG CAG^CTG
181 CAGCTG
RsaI 316 GTAC GT^AC
452 GTAC
StyI 258 CCATGG C^C[AT][AT]GG
444 CCAAGG
TfiI 186 GAATC G^A[AT]TC
392 GAATC
Tsp45I 153 GTCAC ^GT[GC]AC
328 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
HaeIII HgaI HhaI HinP1I HinfI
ItaI MnlI MslI MvaI MwoI
NlaIV Sau96I ScrFI SfaNI TseI
TspRI