Restriction map for
pFN21AE1751
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstF5I BstYI BstZ17I Bsu36I CelII
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FokI
FseI HincII HindII HindIII HpaI
KpnI MaeII MamI MfeI MflI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 418 GTCTAC GT^[AC][GT]AC
AlwI 238 GATCC GGATC(4/5)
AviII 276 TGCGCA TGC^GCA
BalI 404 TGGCCA TGG^CCA
BanI 691 GGCGCC G^G[CT][GA]CC
BbeI 691 GGCGCC GGCGC^C
BfaI 439 CTAG C^TAG
BpmI 413 CTGGAG CTGGAG(16/14)
BseRI 564 CTCCTC GAGGAG(10/8)
BsmAI 204 GAGAC GTCTC(1/5)
BspDI 307 ATCGAT AT^CGAT
BsrI 24 ACTGG ACTGG(1/-1)
BstEII 717 GGTGACC G^GT[ACGT]ACC
BstPI 717 GGTGACC G^GT[ACGT]ACC
BstXI 695 CCACAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 307 ATCGAT AT^CGAT
EarI 709 CTCTTC CTCTTC(1/4)
EcoNI 258 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 717 GGTGACC G^GT[ACGT]ACC
FspI 276 TGCGCA TGC^GCA
KasI 691 GGCGCC G^GCGCC
Ksp632I 709 CTCTTC CTCTTC(1/4)
KspI 191 CCGCGG CCGC^GG
MaeI 439 CTAG C^TAG
MluNI 404 TGGCCA TGG^CCA
MscI 404 TGGCCA TGG^CCA
MslI 613 CACCTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 691 GGCGCC GG^CGCC
NspI 269 GCATGT [GA]CATG^[CT]
PflMI 627 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 216 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 499 CTGCAG CTGCA^G
PvuII 280 CAGCTG CAG^CTG
SacII 191 CCGCGG CCGC^GG
SfcI 499 CTGCAG C^T[GA][CT]AG
SfoI 691 GGCGCC GGC^GCC
StuI 339 AGGCCT AGG^CCT
TaqI 308 TCGA T^CGA
TthHB8I 308 TCGA T^CGA
Van91I 627 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 637 GGCGTC G[GA]^CG[CT]C
691 GGCGCC
AfaI 76 GTAC GT^AC
327 GTAC
AlwNI 502 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
628 CAGCGCCTG
ApaI 86 GGGCCC GGGCC^C
313 GGGCCC
AvaI 201 CCCGAG C^[CT]CG[GA]G
220 CCCGAG
BbsI 139 GTCTTC GAAGAC(2/6)
427 GTCTTC
BglI 341 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
529 GCCGCCCTGGC
BpuAI 139 GTCTTC GAAGAC(2/6)
427 GTCTTC
BsaHI 637 GGCGTC G[GA]^CG[CT]C
691 GGCGCC
BsgI 493 CTGCAC GTGCAG(16/14)
610 CTGCAC
BsoBI 201 CCCGAG C^[CT]CG[GA]G
220 CCCGAG
BstAPI 501 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
664 GCACGCCATGC
DpnII 238 GATC ^GATC
306 GATC
EcoT14I 82 CCTTGG C^C[AT][AT]GG
407 CCAAGG
HaeII 629 AGCGCC [GA]GCGC^[CT]
691 GGCGCC
HgaI 16 GACGC GACGC(5/10)
638 GCGTC
Hin1I 637 GGCGTC G[GA]^CG[CT]C
691 GGCGCC
HphI 717 GGTGA GGTGA(8/7)
748 TCACC
MaeIII 332 GTGAC ^GT[ACGT]AC
718 GTGAC
MboI 238 GATC ^GATC
306 GATC
MspA1I 191 CCGCGG C[AC]G^C[GT]G
280 CAGCTG
NdeII 238 GATC ^GATC
306 GATC
NlaIII 270 CATG CATG^
670 CATG
RsaI 76 GTAC GT^AC
327 GTAC
Sau3AI 238 GATC ^GATC
306 GATC
StyI 82 CCTTGG C^C[AT][AT]GG
407 CCAAGG
Tsp45I 332 GTGAC ^GT[GC]AC
718 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BspMI BsrFI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboII MnlI MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIV PleI Sau96I ScrFI
TseI TspRI