Restriction map for
pFN21AE6892
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
AspEI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI HincII HindII
HindIII HpaI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tsp509I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflIII 659 ACGTGT A^C[GA][CT]GT
Alw44I 322 GTGCAC G^TGCAC
Aor51HI 389 AGCGCT AGC^GCT
ApaI 822 GGGCCC GGGCC^C
ApaLI 322 GTGCAC G^TGCAC
AspHI 322 GTGCAC G[AT]GC[AT]^C
AviII 1058 TGCGCA TGC^GCA
AvrII 133 CCTAGG C^CTAGG
BalI 31 TGGCCA TGG^CCA
BbeI 38 GGCGCC GGCGC^C
BbrPI 874 CACGTG CAC^GTG
BfaI 134 CTAG C^TAG
BlnI 133 CCTAGG C^CTAGG
BsaAI 874 CACGTG [CT]AC^GT[GA]
BsgI 14 GTGCAG GTGCAG(16/14)
BsiEI 285 CGACCG CG[GA][CT]^CG
BsiHKAI 322 GTGCAC G[AT]GC[AT]^C
BsrBI 547 CCGCTC CCGCTC(-3/-3)
BstAPI 1013 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 896 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 389 AGCGCT AGC^GCT
EcoNI 684 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 334 GATATC GAT^ATC
FspI 1058 TGCGCA TGC^GCA
KasI 38 GGCGCC G^GCGCC
MaeI 134 CTAG C^TAG
MaeIII 354 GTCAC ^GT[ACGT]AC
MluNI 31 TGGCCA TGG^CCA
MscI 31 TGGCCA TGG^CCA
NarI 38 GGCGCC GG^CGCC
NcoI 34 CCATGG C^CATGG
PflMI 429 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 874 CACGTG CAC^GTG
PmlI 874 CACGTG CAC^GTG
SfaNI 118 GATGC GCATC(5/9)
SfoI 38 GGCGCC GGC^GCC
SmlI 649 CTCAAG C^T[CT][GA]AG
TaqI 740 TCGA T^CGA
TfiI 1039 GAATC G^A[AT]TC
Tsp45I 354 GTCAC ^GT[GC]AC
TthHB8I 740 TCGA T^CGA
Van91I 429 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 911 GGTACC G^GTACC
1046 GGTACC
AcyI 38 GGCGCC G[GA]^CG[CT]C
862 GACGCC
Asp718I 911 GGTACC G^GTACC
1046 GGTACC
AspI 310 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
708 GACGCTGTC
AvaI 110 CCCGAG C^[CT]CG[GA]G
372 CCCGAG
BamHI 3 GGATCC G^GATCC
127 GGATCC
BpmI 514 CTGGAG CTGGAG(16/14)
793 CTGGAG
BsaHI 38 GGCGCC G[GA]^CG[CT]C
862 GACGCC
BsoBI 110 CCCGAG C^[CT]CG[GA]G
372 CCCGAG
BspMI 98 ACCTGC ACCTGC(4/8)
914 ACCTGC
BstF5I 219 CATCC GGATG(2/0)
870 CATCC
FokI 219 CATCC GGATG(9/13)
870 CATCC
HaeII 38 GGCGCC [GA]GCGC^[CT]
389 AGCGCT
Hin1I 38 GGCGCC G[GA]^CG[CT]C
862 GACGCC
KpnI 911 GGTACC GGTAC^C
1046 GGTACC
MaeII 659 ACGT A^CGT
875 ACGT
MboII 89 TCTTC GAAGA(8/7)
494 TCTTC
MslI 219 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
870 CATCCACGTG
PflFI 310 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
708 GACGCTGTC
PleI 484 GACTC GAGTC(4/5)
607 GACTC
PpuMI 148 GGGTCCT [GA]G^G[AT]CC[CT]
899 AGGACCT
PvuII 67 CAGCTG CAG^CTG
993 CAGCTG
SbfI 636 CCTGCAGG CCTGCA^GG
915 CCTGCAGG
Sse8387I 636 CCTGCAGG CCTGCA^GG
915 CCTGCAGG
StuI 797 AGGCCT AGG^CCT
1016 AGGCCT
Tth111I 310 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
708 GACGCTGTC
XcmI 25 CCACAATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
563 CCAAGACCGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaJI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrI
BssKI BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI StyI TseI TspRI
XhoII